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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NDUFA9 Recombinant monoclonal antibody (87143-2-RR)

    WB analysis using 87143-2-RR (same clone as 87143-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87143-2-RR (NDUFA9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87143-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of Human lung cancer （Adenocarcinoma） using NDUFA9 Recombinant monoclonal antibody (87143-2-RR)

    IHC staining of Human lung cancer （Adenocarcinoma） using 87143-2-RR (same clone as 87143-2-PBS)

    Immunohistochemical analysis of paraffin-embedded Human lung cancer (Adenocarcinoma) slide using 87143-2-RR (NDUFA9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87143-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using NDUFA9 Recombinant monoclonal antibody (87143-2-RR)

    IHC staining of mouse heart using 87143-2-RR (same clone as 87143-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 87143-2-RR (NDUFA9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87143-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NDUFA9 Recombinant monoclonal antibody (87143-2-RR)

    IF Staining of HeLa using 87143-2-RR (same clone as 87143-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NDUFA9 antibody (87143-2-RR, Clone: 252374E1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 87143-2-PBS in a different storage buffer formulation.

NDUFA9 Recombinant monoclonal antibody, PBS Only

NDUFA9 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 87143-2-PBS
Clone No.252374E1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

CI 39kD, CI-39kD, Complex I-39kD, NADH-ubiquinone oxidoreductase 39 kDa subunit, NDUFS2L

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

87143-2-PBS targets NDUFA9 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag16832

Product name: Recombinant human NDUFA9 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-273 aa of BC009311

Sequence: MAAAAQSRVVRVLSMSRSAITAIATSVCHGPPCRQLHHALMPHGKGGRSSVSGIVATVFGATGFLGRYVVNHLGRMGSQVIIPYRCDKYDIMHLRPMGDLGQLLFLEWDARDKDSIRRVVQHSNVVINLIGRDWETKNFDFEDVFVKIPQAIAQLSKEAGVEKFIHVSHLNANIKSSSRYLRNKAVGEKVVRDAFPEAIIVKPSDIFGREDRFLNSFASMHRFGPIPLGSLGWKTVKQPVYVVDVSKGIVNAVKDPDANGKSFAFVGPSRYLL

相同性解析による交差性が予測される生物種
Full Name NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 9, 39kDa
Calculated molecular weight 377 aa, 43 kDa
Observed molecular weight35 kDa
GenBank accession numberBC009311
Gene Symbol NDUFA9
Gene ID (NCBI) 4704
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ16795
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

NDUFA9 (NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial) is also named as NDUFS2L, CI-39kD and belongs to the complex I NDUFA9 subunit family. It is involved in stabilizing the junction between membrane and matrix arms of complex I, a late assembly step critical for complex I biogenesis and activity.

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