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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NDUFA6 Polyclonal antibody (15445-1-AP)

    WB analysis using 15445-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15445-1-AP (NDUFA6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using NDUFA6 Polyclonal antibody (15445-1-AP)

    WB analysis of human heart using 15445-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 15445-1-AP (NDUFA6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using NDUFA6 Polyclonal antibody (15445-1-AP)

    WB analysis of human heart using 15445-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 15445-1-AP (NDUFA6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using NDUFA6 Polyclonal antibody (15445-1-AP)

    IHC staining of human lung cancer using 15445-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15445-1-AP (NDUFA6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using NDUFA6 Polyclonal antibody (15445-1-AP)

    IHC staining of human lung cancer using 15445-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15445-1-AP (NDUFA6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

NDUFA6 Polyclonal antibody

NDUFA6 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15445-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

B14, CI B14, Complex I B14, LYR motif containing protein 6, LYRM6, NADHB14, NDUFA6

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse heart tissue, human heart tissue, rat heart tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15445-1-AP targets NDUFA6 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag7593

Product name: Recombinant human NDUFA6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 4-128 aa of BC002772

Sequence: SGVRQATSTASTFVKPIFSRDMNEAKRRVRELYRAWYREVPNTVHQFQLDITVKMGRDKVREMFMKNAHVTDPRVVDLLVIKGKIELEETIKVWKQRTHVMRFFHETEAPRPKDFLSKFYVGHDP

相同性解析による交差性が予測される生物種
Full Name NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 6, 14kDa
Calculated molecular weight 15 kDa
Observed molecular weight 15 kDa
GenBank accession numberBC002772
Gene Symbol NDUFA6
Gene ID (NCBI) 4700
RRIDAB_2267065
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP56556
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NDUFA6 (NADH:Ubiquinone Oxidoreductase Subunit A6), also called LYR motif-containing protein 6 (LYRM6), or Complex I-B14 (CI-B14), or NADH-ubiquinone oxidoreductase B14 subunit, is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I). NDUFA6 participates in the regulation of adipogenic differentiation.

Protocols

Product Specific Protocols
WB protocol for NDUFA6 antibody 15445-1-APDownload protocol
IHC protocol for NDUFA6 antibody 15445-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols