Validation Data Gallery
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Tested Applications
| Positive WB detected in | rat brain tissue, mouse liver tissue, mouse heart tissue, rat liver tissue |
| Positive IP detected in | mouse liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 9 publications below |
| IF | See 1 publications below |
| FC | See 1 publications below |
Product Information
26203-1-AP targets NDST1 in WB, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag24422 Product name: Recombinant human NDST1 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 38-90 aa of BC012888 Sequence: LYGWKRGLEPSADAPEPDCGDPPPVAPSRLLPLKPVQAATPSRTDPLVLVFVE 相同性解析による交差性が予測される生物種 |
| Full Name | N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 1 |
| Calculated molecular weight | 882 aa, 101 kDa |
| Observed molecular weight | 68-70 kDa |
| GenBank accession number | BC012888 |
| Gene Symbol | NDST1 |
| Gene ID (NCBI) | 3340 |
| RRID | AB_2880423 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P52848 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for NDST1 antibody 26203-1-AP | Download protocol |
| WB protocol for NDST1 antibody 26203-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model | ||
J Extracell Vesicles Quantification of urinary podocyte-derived migrasomes for the diagnosis of kidney disease | ||
Adv Sci (Weinh) Bone Marrow Mesenchymal Stem Cell-Derived Dermcidin-Containing Migrasomes enhance LC3-Associated Phagocytosis of Pulmonary Macrophages and Protect against Post-Stroke Pneumonia | ||
Exp Mol Med PGC-1α mediates migrasome secretion accelerating macrophage-myofibroblast transition and contributing to sepsis-associated pulmonary fibrosis | ||
Ann Neurol Parkinson's Disease Derived Exosomes Aggravate Neuropathology in SNCA*A53T Mice.
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PLoS Pathog N-Glycans and sulfated glycosaminoglycans contribute to the action of diverse Tc toxins on mammalian cells.
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