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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NDRG3 Polyclonal antibody (31421-1-AP)

    WB analysis using 31421-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31421-1-AP (NDRG3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using NDRG3 Polyclonal antibody (31421-1-AP)

    IHC staining of human colon using 31421-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 31421-1-AP (NDRG3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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NDRG3 Polyclonal antibody

NDRG3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 31421-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

Protein NDRG3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, Raji cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31421-1-AP targets NDRG3 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NDRG3 fusion protein Ag33626 相同性解析による交差性が予測される生物種
Full Name NDRG family member 3
Calculated molecular weight41 kDa
Observed molecular weight41-45 kDa
GenBank accession numberNM_032013
Gene Symbol NDRG3
Gene ID (NCBI) 57446
RRIDAB_3669974
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9UGV2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NDRG (N-Myc downstream-regulated gene) family is generally divided into two subfamilies, one composed of NDRG1 and NDRG3 with a homology of 67%, and the other composed of NDRG2 and NDRG4 with a homology of 58%. NDRG3 is a downstream regulatory factor of MYC, with a total length of 2588 base pairs. It is mainly expressed in ovary, prostate, testis, brain, spinal cord, thymus, heart and kidney. NDRG3 is located on chromosome 20q11.21-11.23 and encodes at least two subtypes, 375 and 363 amino acids, respectively, with an apparent molecular weight of 41 and 40 kDa, respectively. (PMID: 36619553)

Protocols

Product Specific Protocols
WB protocol for NDRG3 antibody 31421-1-APDownload protocol
IHC protocol for NDRG3 antibody 31421-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols