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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NDRG1 Recombinant monoclonal antibody (85141-4-RR)

    WB analysis using 85141-4-RR (same clone as 85141-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85141-4-RR (NDRG1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85141-4-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using NDRG1 Recombinant monoclonal antibody (85141-4-RR)

    IP experiment of HeLa using 85141-4-RR (same clone as 85141-4-PBS)

    IP result of anti-NDRG1 (IP:85141-4-RR, 4ug; Detection:85141-4-RR 1:1000) with HeLa cells lysate 1520 ug. This data was developed using the same antibody clone with 85141-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using NDRG1 Recombinant monoclonal antibody (85141-4-RR)

    IHC staining of human lung cancer using 85141-4-RR (same clone as 85141-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 85141-4-RR (NDRG1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85141-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using NDRG1 Recombinant monoclonal antibody (85141-4-RR)

    IHC staining of human lung cancer using 85141-4-RR (same clone as 85141-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 85141-4-RR (NDRG1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85141-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using NDRG1 Recombinant monoclonal antibody (85141-4-RR)

    FC experiment of HeLa using 85141-4-RR (same clone as 85141-4-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug NDRG1 Recombinant antibody (85141-4-RR, Clone:242322F7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 85141-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85141-4-RR

    Affinity and Kinetic Characterization of 85141-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85141-4-RR against Human NDRG1 were performed. The affinity constant is below 1 pM.

NDRG1 Recombinant monoclonal antibody, PBS Only

NDRG1 Uni-rAb® Recombinant Antibody for WB, IHC, FC (Intra), IP, Indirect ELISA
Cat No. 85141-4-PBS
Clone No.242322F7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), IP, Indirect ELISA

Synonyms

242322F7, CMT4D, Differentiation-related gene 1 protein, DRG1, DRG-1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85141-4-PBS targets NDRG1 in WB, IHC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag25424

Product name: Recombinant human NDRG1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 45-109 aa of BC006260

Sequence: SGSSVTSLDGTRSRSHTSEGTRSRSHTSEGTRSRSHTSEGAHLDITPNSGAAGNSAGPKSMEVSC

相同性解析による交差性が予測される生物種
Full Name N-myc downstream regulated 1
Calculated molecular weight 43 kDa
Observed molecular weight45-46 kDa
GenBank accession numberBC006260
Gene Symbol NDRG1
Gene ID (NCBI) 10397
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ92597
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

NDRG1 is a member of the N-myc down-regulated gene family which belongs to the alpha/beta hydrolase superfamily. NDRG1 is a highly conserved cytoplasmic protein involved in cell growth arrest and proliferation, cell differentiation, DNA damage response, heavy metal response, the hypoxia response, tumorigenesis, and metastasis. NDRG1 is a potent metastasis suppressor in multiple tumor-types. NDRG1 appears to serve as a putative tumor suppressor and is down-regulated in colorectal, gastric, cervical, ovarian and prostate cancers.

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