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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NDP52 Recombinant antibody (86167-1-RR)

    WB analysis using 86167-1-RR (same clone as 86167-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86167-1-RR (NDP52 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86167-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NDP52 Recombinant antibody (86167-1-RR)

    IF Staining of HeLa using 86167-1-RR (same clone as 86167-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NDP52 antibody (86167-1-RR, Clone: 250714E9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86167-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86167-1-RR

    Affinity and Kinetic Characterization of 86167-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86167-1-RR against Human NDP52 were performed. The affinity constant is 0.643 nM.

NDP52 Recombinant monoclonal antibody, PBS Only

NDP52 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86167-1-PBS
Clone No.250714E9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

CALCOCO2, Calcium-binding and coiled-coil domain-containing protein 2, Nuclear domain 10 protein 52, Nuclear dot protein 52

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86167-1-PBS targets NDP52 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag2866

Product name: Recombinant human CALCOCO2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-283 aa of BC015893

Sequence: MEETIKDPPTSAVLLDHCHFSQVIFNSVEKFYIPGGDVTCHYTFTQHFIPRRKDWIGIFRVGWKTTREYYTFMWVTLPIDLNNKSAKQQEVQFKAYYLPKDDEYYQFCYVDEDGVVRGASIPFQFRPENEEDILVVTTQGEVEEIEQHNKELCKENQELKDSCISLQKQNSDMQAELQKKQEELETLQSINKKLELKVKEQKDYWETELLQLKEQNQKMSSENEKMGIRVDQLQAQLSTQEKEMEKLVQGDQDKTEQLEQLKKENDHLFLSLTEQRKDQKKLE

相同性解析による交差性が予測される生物種
Full Name calcium binding and coiled-coil domain 2
Calculated molecular weight 446 aa, 52 kDa
Observed molecular weight52 kDa
GenBank accession numberBC015893
Gene Symbol NDP52
Gene ID (NCBI) 10241
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ13137
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

NDP52, also named as CALCOCO2, is an autophagy receptor. It plays a role in ruffle formation and actin cytoskeleton organization. Mouse/Rat NDP52 has some isoforms with MW 28-40 kDa and 67 kDa. Human NDP52 has some isoforms with MW 43-47 kDa and 52-55 kDa.

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