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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NDFIP2 Polyclonal antibody (32043-1-AP)

    WB analysis using 32043-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32043-1-AP (NDFIP2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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NDFIP2 Polyclonal antibody

NDFIP2 Polyclonal Antibody for WB, ELISA
Cat No. 32043-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Putative MAPK-activating protein PM04/PM05/PM06/PM07, NEDD4 WW domain-binding protein 5A, NEDD4 family-interacting protein 2, Nedd4 Family Interacting Protein 2, N4WBP5A

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA431 cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32043-1-AP targets NDFIP2 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NDFIP2 fusion protein Ag36480 相同性解析による交差性が予測される生物種
Full Name Nedd4 family interacting protein 2
Calculated molecular weight36kDa，336aa
Observed molecular weight39 kDa
GenBank accession numberNM_019080.2
Gene Symbol NDFIP2
Gene ID (NCBI) 54602
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9NV92
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NDFIP2 (Nedd4 family interacting protein 2) is a gene encoding a membrane-anchored adapter protein involved in a variety of cellular processes. It regulates protein ubiquitination and degradation by binding to the Nedd4 family of E3 ubiquitin ligases.NDFIP2 has a wide intracellular distribution, including the Golgi, mitochondria, and perinuclear region of the cytoplasm. Its function involves negative regulation of gene expression, negative regulation of protein transport, and positive regulation of protein ubiquitination. In addition, NDFIP2 is associated with a variety of diseases, such as supratentorial ventricular meningiomas in children. In the cardiovascular field, NDFIP2 affects cardiac electrophysiologic function by regulating the degradation of hERG potassium channels. It is also involved in the immune response, affecting T cell function by regulating cytokine signaling pathways. In oncology, NDFIP2 has been found to affect the antiviral response by targeting IFITM3.

Protocols

Product Specific Protocols
WB protocol for NDFIP2 antibody 32043-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols