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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NCX1 Polyclonal antibody (28447-1-AP)

    WB analysis using 28447-1-AP (same clone as 28447-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28447-1-AP (NCX1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28447-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using NCX1 Polyclonal antibody (28447-1-AP)

    WB analysis using 28447-1-AP (same clone as 28447-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28447-1-AP (NCX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28447-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using NCX1 Polyclonal antibody (28447-1-AP)

    IHC staining of human kidney using 28447-1-AP (same clone as 28447-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28447-1-AP (NCX1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28447-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using NCX1 Polyclonal antibody (28447-1-AP)

    IHC staining of human kidney using 28447-1-AP (same clone as 28447-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28447-1-AP (NCX1 antibody) at dilution of 1:1600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28447-1-PBS in a different storage buffer formulation.

NCX1 Polyclonal antibody, PBS Only

NCX1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 28447-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Na(+)/Ca(2+)-exchange protein 1, NCX 1, NCX-1, SLC8A1, Solute carrier family 8 member 1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

28447-1-PBS targets NCX1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag28952

Product name: Recombinant human SLC8A1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-59 aa of NM_021097

Sequence: MYNMRRLSLSPTFSMGFHLLVTVSLLFSHVDHVIAETEMEGEGNETGECTGSYYCKKGV

相同性解析による交差性が予測される生物種
Full Name solute carrier family 8 (sodium/calcium exchanger), member 1
Calculated molecular weight 109 kDa
Observed molecular weight70 kDa
GenBank accession numberNM_021097
Gene Symbol NCX1
Gene ID (NCBI) 6546
RRIDAB_2918167
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP32418
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SLC8A1, also known as NCX1, belongs to the SLC (Solute Carrier) family and the CaCA (Calcium/Cation Antiporter) superfamily. NCX1 is a bidirectional ion exchanger that can exchange sodium and calcium ions between the inside and outside of cells in a 1:3 ratio (1 Ca2+ ion and 3 Na+ ions). Under physiological conditions, NCX1 mainly transports Ca2+ from the cell interior to the exterior through the forward transport mode to maintain the low calcium level inside the cell. However, under certain special conditions, such as ischemia-reperfusion injury and stroke, the reverse transport mode of NCX1 is activated, leading to the influx of Ca2+ into the cell, which may cause cell damage (PMID: 21289289, 30686898).

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