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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NCOR2 Polyclonal antibody (29952-1-AP)

    WB analysis using 29952-1-AP (same clone as 29952-1-PBS)

    MOLT-4 cells were subjected to Tris-Acetate gel system followed by western blot with 29952-1-AP (NCOR2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29952-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using NCOR2 Polyclonal antibody (29952-1-AP)

    WB analysis using 29952-1-AP (same clone as 29952-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29952-1-AP (NCOR2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29952-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using NCOR2 Polyclonal antibody (29952-1-AP)

    IHC staining of human prostate cancer using 29952-1-AP (same clone as 29952-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 29952-1-AP (NCOR2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29952-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using NCOR2 Polyclonal antibody (29952-1-AP)

    IHC staining of human prostate cancer using 29952-1-AP (same clone as 29952-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 29952-1-AP (NCOR2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29952-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using NCOR2 Polyclonal antibody (29952-1-AP)

    IF Staining of A549 using 29952-1-AP (same clone as 29952-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using NCOR2 antibody (29952-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 29952-1-PBS in a different storage buffer formulation.

NCOR2 Polyclonal antibody, PBS Only

NCOR2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 29952-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29952-1-PBS targets NCOR2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag30727

Product name: Recombinant human NCOR2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1165-1363 aa of NM_006312

Sequence: SGVKQEQLSPRGQAGPPESLGVPTAQEASVLRGTALGSVPGGSITKGIPSTRVPSDSAITYRGSITHGTPADVLYKGTITRIIGEDSPSRLDRGREDSLPKGHVIYEGKKGHVLSYEGGMSVTQCSKEDGRSSSGPPHETAAPKRTYDMMEGRVGRAISSASIEGLMGRAIPPERHSPHHLKEQHHIRGSITQGIPRSY

相同性解析による交差性が予測される生物種
Full Name nuclear receptor co-repressor 2
Calculated molecular weight 274 kDa
Observed molecular weight274 kDa
GenBank accession numberNM_006312
Gene Symbol NCOR2
Gene ID (NCBI) 9612
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y618
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

NCOR2, nuclear receptor corepressor 2 , is a transcriptional corepressor that that contains several nuclear receptor-interacting domains. NCOR2/SMRT acts as a platform protein, facilitating the recruitment of histone deacetylases to the DNA promoters bound by its interacting transcription factors (PMID: 9150137). NCOR2 mediates the transcriptional repression activity of some nuclear receptors by promoting chromatin condensation, thus preventing access of the basal transcription (PMID: 23081895).

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