• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Use Able AI chat for product recommendations

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using NCOA5 Polyclonal antibody (20175-1-AP)

    WB analysis using 20175-1-AP (same clone as 20175-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20175-1-AP (NCOA5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20175-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using NCOA5 Polyclonal antibody (20175-1-AP)

    IP experiment of HEK-293 using 20175-1-AP (same clone as 20175-1-PBS)

    IP result of anti-NCOA5 (IP:20175-1-AP, 4ug; Detection:20175-1-AP 1:800) with HEK-293 cells lysate 3000ug. This data was developed using the same antibody clone with 20175-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using NCOA5 Polyclonal antibody (20175-1-AP)

    IHC staining of human stomach using 20175-1-AP (same clone as 20175-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 20175-1-AP (NCOA5 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20175-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using NCOA5 Polyclonal antibody (20175-1-AP)

    IHC staining of mouse testis using 20175-1-AP (same clone as 20175-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20175-1-AP (NCOA5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20175-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using NCOA5 Polyclonal antibody (20175-1-AP)

    IHC staining of mouse testis using 20175-1-AP (same clone as 20175-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20175-1-AP (NCOA5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20175-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using NCOA5 Polyclonal antibody (20175-1-AP)

    IF Staining of A431 using 20175-1-AP (same clone as 20175-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using NCOA5 antibody (20175-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 20175-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HEK-293 cells using NCOA5 Polyclonal antibody (20175-1-AP)

    FC experiment of HEK-293 using 20175-1-AP (same clone as 20175-1-PBS)

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug NCOA5 Polyclonal antibody (20175-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 20175-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

NCOA5 Polyclonal antibody, PBS Only

NCOA5 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 20175-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Synonyms

CIA, Coactivator independent of AF-2, KIAA1637, NCoA-5, Nuclear receptor coactivator 5

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20175-1-PBS targets NCOA5 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag14014

Product name: Recombinant human NCOA5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 112-384 aa of BC056872

Sequence: DMRDSRDPMYRREGSYDRYLRMDDYCRRKDDSYFDRYRDSFDGRGPPGPESQSRAKERLKREERRREELYRQYFEEIQRRFDAERPVDCSVIVVNKQTKDYAESVGRKVRDLGMVVDLIFLNTEVSLSQALEDVSRGGSPFAIVITQQHQIHRSCTVNIMFGTPQEHRNMPQADAMVLVARNYERYKNECREKEREEIARQAAKMADEAILQERERGGPEEGVRGGHPPAIQSLINLLADNRYLTAEETDKIINYLRERKERLMRSSTDSLPG

相同性解析による交差性が予測される生物種
Full Name nuclear receptor coactivator 5
Calculated molecular weight 579 aa, 66 kDa
Observed molecular weight 66 kDa
GenBank accession numberBC056872
Gene Symbol NCOA5
Gene ID (NCBI) 57727
RRIDAB_10694674
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HCD5
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Nuclear receptor coactivator 5 (NCOA5), also known as coactivator independent of AF-2 (CIA), possesses both repressor and activator functions (PMID: 11113208). It can interact with Estrogen Receptor (ER) alpha and ER beta in a ligand-dependent manner. NCOA5 has been shown to regulate the ER alpha-mediated transcription as well as the expression of the c-Myc gene (PMID: 11113208; 15073177). Recently identified as a conserved component of pluripotent stem cells, mammalian NCOA5 may contribute to the maintenance of the pluripotent stem cell population (PMID: 24268775).

||
New chat

Able™

正在加载，请稍候...