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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NCOA3 Polyclonal antibody (29587-1-AP)

    WB analysis using 29587-1-AP (same clone as 29587-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29587-1-AP (NCOA3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29587-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using NCOA3 Polyclonal antibody (29587-1-AP)

    WB analysis using 29587-1-AP (same clone as 29587-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29587-1-AP (NCOA3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29587-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using NCOA3 Polyclonal antibody (29587-1-AP)

    IHC staining of human lung cancer using 29587-1-AP (same clone as 29587-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29587-1-AP (NCOA3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29587-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using NCOA3 Polyclonal antibody (29587-1-AP)

    IHC staining of human skin cancer using 29587-1-AP (same clone as 29587-1-PBS)

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 29587-1-AP (NCOA3 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29587-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using NCOA3 Polyclonal antibody (29587-1-AP)

    IHC staining of human lung cancer using 29587-1-AP (same clone as 29587-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29587-1-AP (NCOA3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29587-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NCOA3 Polyclonal antibody (29587-1-AP)

    IF Staining of HeLa using 29587-1-AP (same clone as 29587-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NCOA3 antibody (29587-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 29587-1-PBS in a different storage buffer formulation.

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NCOA3 Polyclonal antibody, PBS Only

NCOA3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 29587-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

AIB1, SRC3, ACTR, AIB 1, AIB-1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29587-1-PBS targets NCOA3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NCOA3 fusion protein Ag30633 相同性解析による交差性が予測される生物種
Full Name nuclear receptor coactivator 3
Calculated molecular weight 1424 aa, 155 kDa
Observed molecular weight150-160 kDa
GenBank accession numberBC119001
Gene Symbol NCOA3
Gene ID (NCBI) 8202
RRIDAB_2918328
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y6Q9
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

NCOA3, also named as AIB1, SRC3 and TRAM1, belongs to the SRC/p160 nuclear receptor coactivator family. NCOA3 is a nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. NOCA3 plays a central role in creating a multisubunit coactivator complex, which probably acts via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). NCOA3 also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit.