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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NCOA2/SRC2 Polyclonal antibody (30962-1-AP)

    WB analysis using 30962-1-AP (same clone as 30962-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30962-1-AP (NCOA2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30962-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using NCOA2/SRC2 Polyclonal antibody (30962-1-AP)

    IF Staining of U2OS using 30962-1-AP (same clone as 30962-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using NCOA2 antibody (30962-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 30962-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NCOA2/SRC2 Polyclonal antibody (30962-1-AP)

    IF Staining of HepG2 using 30962-1-AP (same clone as 30962-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NCOA2 antibody (30962-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 30962-1-PBS in a different storage buffer formulation.

NCOA2/SRC2 Polyclonal antibody, PBS Only

NCOA2/SRC2 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 30962-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

NCOA2, bHLHe75, Class E basic helix-loop-helix protein 75, GRIP1, hTIF2

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

30962-1-PBS targets NCOA2/SRC2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag33768

Product name: Recombinant human NCOA2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 550-820 aa of BC114383

Sequence: SSLASPDLKMGNLQNSPVNMNPPPLSKMGSLDSKDCFGLYGEPSEGTTGQAESSCHPGEQKETNDPNLPPAVSSERADGQSRLHDSKGQTKLLQLLTTKSDQMEPSPLASSLSDTNKDSTGSLPGSGSTHGTSLKEKHKILHRLLQDSSSPVDLAKLTAEATGKDLSQESSSTAPGSEVTIKQEPVSPKKKENALLRYLLDKDDTKDIGLPEITPKLERLDSKTDPASNTKLIAMKTEKEEMSFEPGDQPGSELDNLEEILDDLQNSQLPQ

相同性解析による交差性が予測される生物種
Full Name nuclear receptor coactivator 2
Calculated molecular weight 1464 aa, 159 kDa
Observed molecular weight160-180 kDa
GenBank accession numberBC114383
Gene Symbol NCOA2
Gene ID (NCBI) 10499
RRIDAB_3669796
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ15596
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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