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  • Featured Product
  • KD/KO Validated

NCKIPSD Polyclonal antibody

NCKIPSD Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11367-1-AP

Synonyms

AF3P21, Dia interacting protein 1, DIP, DIP 1, DIP1, NCKIPSD, ORF1, SH3 adapter protein SPIN90, SPIN90, VIP54, WASLBP, WISH

Validation Data Gallery

Applications:
  • WB analysis of mouse brain using 11367-1-AP

    WB analysis of mouse brain using 11367-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 11367-1-AP (NCKIPSD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of human heart using 11367-1-AP

    WB analysis of human heart using 11367-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 11367-1-AP (NCKIPSD antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • WB analysis of fetal human brain using 11367-1-AP

    WB analysis of fetal human brain using 11367-1-AP

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 11367-1-AP (NCKIPSD Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse brain using 11367-1-AP

    WB analysis of mouse brain using 11367-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 11367-1-AP (NCKIPSD Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 11367-1-AP

    IP experiment of mouse brain using 11367-1-AP

    IP result of anti-NCKIPSD (IP:11367-1-AP, 4ug; Detection:11367-1-AP 1:300) with mouse brain tissue lysate 4000ug.

  • IHC staining of human breast cancer using 11367-1-AP

    IHC staining of human breast cancer using 11367-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11367-1-AP (NCKIPSD Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 11367-1-AP

    IHC staining of human breast cancer using 11367-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11367-1-AP (NCKIPSD Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse brain tissue, fetal human brain tissue, human heart tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11367-1-AP targets NCKIPSD in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NCKIPSD fusion protein Ag1915 相同性解析による交差性が予測される生物種
Full Name NCK interacting protein with SH3 domain
Calculated molecular weight 715 aa, 78 kDa
Observed molecular weight 80 kDa
GenBank accession numberBC016052
Gene symbol NCKIPSD
Gene ID (NCBI) 51517
RRIDAB_2150732
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NCKIPSD antibody 11367-1-APDownload protocol
IHC protocol for NCKIPSD antibody 11367-1-APDownload protocol
IP protocol for NCKIPSD antibody 11367-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IHC

Cancer Res

SPIN90 depletion and Microtubule Acetylation Mediate Stromal Fibroblast Activation in Breast Cancer Progression.

Authors - Eunae You
  • KO Validated
View Article