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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using NCKAP5L Polyclonal antibody (21392-1-AP)

    WB analysis of HeLa using 21392-1-AP (same clone as 21392-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 21392-1-AP (NCKAP5L antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21392-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin tissue using NCKAP5L Polyclonal antibody (21392-1-AP)

    IHC staining of human skin using 21392-1-AP (same clone as 21392-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin using 21392-1-AP (NCKAP5L antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 21392-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin tissue using NCKAP5L Polyclonal antibody (21392-1-AP)

    IHC staining of human skin using 21392-1-AP (same clone as 21392-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin using 21392-1-AP (NCKAP5L antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 21392-1-PBS in a different storage buffer formulation.

NCKAP5L Polyclonal antibody, PBS Only

NCKAP5L Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 21392-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21392-1-PBS targets NCKAP5L in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag16050

Product name: Recombinant human KIAA1602 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-61 aa of BC110599

Sequence: MDQPAGGPGNPRPGEGDDGSMEPGTCQELLHRLRELEAENSALAQANENQRETYERCLDEV

相同性解析による交差性が予測される生物種
Full Name KIAA1602
Calculated molecular weight 1334 aa, 139 kDa
Observed molecular weight 145-150 kDa
GenBank accession numberBC110599
Gene Symbol NCKAP5L
Gene ID (NCBI) 57701
RRIDAB_10860259
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HCH0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

NCKAP5L, also named as KIAA1602, has 4 isoforms. The target band is about 139-145 kDa for phosphorylation.

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