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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using NCKAP1 Polyclonal antibody (12140-1-AP)

    WB analysis of mouse brain using 12140-1-AP (same clone as 12140-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 12140-1-AP (NCKAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12140-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using NCKAP1 Polyclonal antibody (12140-1-AP)

    WB analysis using 12140-1-AP (same clone as 12140-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12140-1-AP (NCKAP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12140-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using NCKAP1 Polyclonal antibody (12140-1-AP)

    IHC staining of mouse heart using 12140-1-AP (same clone as 12140-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12140-1-AP (NCKAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12140-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using NCKAP1 Polyclonal antibody (12140-1-AP)

    IHC staining of mouse brain using 12140-1-AP (same clone as 12140-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12140-1-AP (NCKAP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12140-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using NCKAP1 Polyclonal antibody (12140-1-AP)

    IHC staining of mouse brain using 12140-1-AP (same clone as 12140-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12140-1-AP (NCKAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12140-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using NCKAP1 Polyclonal antibody (12140-1-AP)

    IF Staining of mouse brain using 12140-1-AP (same clone as 12140-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NCKAP1 antibody (12140-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12140-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using NCKAP1 Polyclonal antibody (12140-1-AP)

    IF Staining of U-251 using 12140-1-AP (same clone as 12140-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using NCKAP1 antibody (12140-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 12140-1-PBS in a different storage buffer formulation.

NCKAP1 Polyclonal antibody, PBS Only

NCKAP1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 12140-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

HEM2, KIAA0587, Membrane-associated protein HEM-2, NAP 1, NAP1

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12140-1-PBS targets NCKAP1 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2788

Product name: Recombinant human NAP1;NCKAP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 779-1129 aa of BC015025

Sequence: HGEPTITSLYTNWYLETLLRQVSNGHIAYFPAMKAFVNLPTENELTFNAEEYSDISEMRSLSELLGPYGMKFLSESLMWHISSQVAELKKLVVENVDVLTQMRTSFDKPDQMAALFKRLSSVDSVLKRMTIIGVILSFRSLAQEALRDVLSYHIPFLVSSIEDFKDHIPRETDMKVAMNVYELSSAAGLPCEIDPALVVALSSQKSENISPEEEYKIACLLMVFVAVSLPTLASNVMSQYSPAIEGHCNNIHCLAKAINQIAAALFTIHKGSIEDRLKEFLALASSSLLKIGQETDKTTTRNRESVYLLLDMIVQESPFLTMDLLESCFPYVLLRNAYHAVYKQSVTSSA

相同性解析による交差性が予測される生物種
Full Name NCK-associated protein 1
Calculated molecular weight 129 kDa
Observed molecular weight 115-125 kDa
GenBank accession numberBC015025
Gene Symbol NCKAP1
Gene ID (NCBI) 10787
RRIDAB_10666284
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y2A7
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

NCK-associated protein 1 (NCKAP1), also known as NAP1 or HEM2, is encoded by an apoptosis-related gene human NAP1, the expression of which is strongly down-regulated in sporadic Alzheimer's disease (AD). Human NAP1 is predominantly expressed in neuronal cells. Antisense oligo DNA of human NAP1 transcripts was found to induce apoptosis of neuronal cells. Loss of NAP1 function disrupts neuronal differentiation. Likewise, NAP1 plays an essential role in facilitating neuronal cytoskeletal changes underlying the postmigratory differentiation of cortical neurons, a critical step in functional wiring of the cortex. Human NAP1 is also proved to be an orthologue of rat Nap1 which binds to the adaptor molecule NCK in signal transduction.

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