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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NCAPD2 Polyclonal antibody (13382-1-AP)

    WB analysis using 13382-1-AP (same clone as 13382-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 13382-1-AP (NCAPD2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13382-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using NCAPD2 Polyclonal antibody (13382-1-AP)

    WB analysis of COLO 320 using 13382-1-AP (same clone as 13382-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 13382-1-AP (NCAPD2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13382-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using NCAPD2 Polyclonal antibody (13382-1-AP)

    WB analysis of HEK-293 using 13382-1-AP (same clone as 13382-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 13382-1-AP (NCAPD2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13382-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using NCAPD2 Polyclonal antibody (13382-1-AP)

    WB analysis of HeLa using 13382-1-AP (same clone as 13382-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 13382-1-AP (NCAPD2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13382-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using NCAPD2 Polyclonal antibody (13382-1-AP)

    WB analysis of K-562 using 13382-1-AP (same clone as 13382-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 13382-1-AP (NCAPD2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13382-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using NCAPD2 Polyclonal antibody (13382-1-AP)

    IP experiment of HEK-293 using 13382-1-AP (same clone as 13382-1-PBS)

    IP result of anti-NCAPD2 (IP:13382-1-AP, 4ug; Detection:13382-1-AP 1:600) with HEK-293 cells lysate 2800ug. This data was developed using the same antibody clone with 13382-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using NCAPD2 Polyclonal antibody (13382-1-AP)

    IHC staining of human ovary cancer using 13382-1-AP (same clone as 13382-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 13382-1-AP (NCAPD2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13382-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using NCAPD2 Polyclonal antibody (13382-1-AP)

    IHC staining of human skin cancer using 13382-1-AP (same clone as 13382-1-PBS)

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 13382-1-AP (NCAPD2 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13382-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NCAPD2 Polyclonal antibody (13382-1-AP)

    IF Staining of HeLa using 13382-1-AP (same clone as 13382-1-PBS)

    Immunofluorescent analysis of Hela cells, using NCAPD2 antibody 13382-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 13382-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NCAPD2 Polyclonal antibody (13382-1-AP)

    IF Staining of HeLa using 13382-1-AP (same clone as 13382-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 13382-1-AP (NCAPD2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13382-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

NCAPD2 Polyclonal antibody, PBS Only

NCAPD2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 13382-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Condensin complex subunit 1, CNAP1, Chromosome-associated protein D2, Chromosome condensation-related SMC-associated protein 1, CAPD2

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13382-1-PBS targets NCAPD2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag4219

Product name: Recombinant human NCAPD2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-351 aa of BC028182

Sequence: MAPQMYEFHLPLSPEELLKSGGVNQYVVQEVLSIKHLPPQLRAFQAAFRAQGPLAMLQHFDTIYSILHHFRSIDPGLKEDTLEFLIKVVSRHSQELPAILDDTTLSGSDRNAHLNALKMNCYALIRLLESFETMASQTNLVDLDLGGKGKKARTKAAHGFDWEEERQPILQLLTQLLQLDIRHLWNHSIIEEEFVSLVTGCCYRLLENPTINHQKNRPTREAITHLLGVALTRYNHMLSATVKIIQMLQHFEHLAPVLVAAVSLWATDYGMKSIVGEIVREIGQKCPQELSRDPSGTKGFAAFLTELAERVPAILMSSMCILLDHLDGENYMMRNAVLAAMAEMVLQVLSG

相同性解析による交差性が予測される生物種
Full Name non-SMC condensin I complex, subunit D2
Calculated molecular weight 1401 aa, 157 kDa
Observed molecular weight 157 kDa
GenBank accession numberBC028182
Gene Symbol NCAPD2
Gene ID (NCBI) 9918
RRIDAB_2149840
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15021
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

NCAPD2 is a regulatory subunit of the condensin complex, which is required for conversion of interphase chromatin into mitotic-like condense chromosomes.  Condensin complexes are heteropentamers comprised of two SMC (structural maintenance of chromosomes) subunits and three non-SMC subunits. The C-terminus of NCAPD2 interacts with Histones H1 and H3 through their histone tails. A loss of NCAPD2 can lead to the disorganization of chromatid axes, misalignment of sister chromatids during metaphase and delayed entry into anaphase.

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