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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NCALD Monoclonal antibody (66088-1-Ig)

    WB analysis using 66088-1-Ig (same clone as 66088-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66088-1-Ig (NCALD antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66088-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using NCALD Monoclonal antibody (66088-1-Ig)

    WB analysis using 66088-1-Ig (same clone as 66088-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66088-1-Ig (NCALD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66088-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using NCALD Monoclonal antibody (66088-1-Ig)

    WB analysis of human brain using 66088-1-Ig (same clone as 66088-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 66088-1-Ig (NCALD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66088-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using NCALD Monoclonal antibody (66088-1-Ig)

    WB analysis using 66088-1-Ig (same clone as 66088-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66088-1-Ig (NCALD antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66088-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using NCALD Monoclonal antibody (66088-1-Ig)

    IHC staining of human cerebellum using 66088-1-Ig (same clone as 66088-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cerebellum using 66088-1-Ig(NCALD antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66088-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using NCALD Monoclonal antibody (66088-1-Ig)

    IHC staining of human testis using 66088-1-Ig (same clone as 66088-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis slide using 66088-1-Ig (NCALD Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66088-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using NCALD Monoclonal antibody (66088-1-Ig)

    IHC staining of human testis using 66088-1-Ig (same clone as 66088-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis slide using 66088-1-Ig (NCALD Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66088-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using NCALD Monoclonal antibody (66088-1-Ig)

    IHC staining of mouse brain using 66088-1-Ig (same clone as 66088-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66088-1-Ig (NCALD antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66088-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using NCALD Monoclonal antibody (66088-1-Ig)

    IHC staining of mouse brain using 66088-1-Ig (same clone as 66088-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66088-1-Ig (NCALD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66088-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using NCALD Monoclonal antibody (66088-1-Ig)

    IHC staining of mouse cerebellum using 66088-1-Ig (same clone as 66088-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66088-1-Ig (NCALD antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66088-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using NCALD Monoclonal antibody (66088-1-Ig)

    IHC staining of mouse cerebellum using 66088-1-Ig (same clone as 66088-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66088-1-Ig (NCALD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66088-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using NCALD Monoclonal antibody (66088-1-Ig)

    IHC staining of mouse cerebellum using 66088-1-Ig (same clone as 66088-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66088-1-Ig (NCALD antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66088-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using NCALD Monoclonal antibody (66088-1-Ig)

    IHC staining of mouse cerebellum using 66088-1-Ig (same clone as 66088-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66088-1-Ig (NCALD antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66088-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using NCALD Monoclonal antibody (66088-1-Ig)

    IHC staining of human cerebellum using 66088-1-Ig (same clone as 66088-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cerebellum using 66088-1-Ig(NCALD antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66088-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NCALD Monoclonal antibody (66088-1-Ig)

    IF Staining of HeLa using 66088-1-Ig (same clone as 66088-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66088-1-Ig (NCALD antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66088-1-PBS in a different storage buffer formulation.

NCALD Monoclonal antibody, PBS Only

NCALD Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66088-1-PBS
Clone No.6B4E10

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, pig, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

NCALD, neurocalcin delta, neurocalcin δ

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66088-1-PBS targets NCALD in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, pig, rat samples.

Tested Reactivity human, mouse, pig, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag18760

Product name: Recombinant human NCALD protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-193 aa of BC036098

Sequence: MGKQNSKLRPEVMQDLLESTDFTEHEIQEWYKGFLRDCPSGHLSMEEFKKIYGNFFPYGDASKFAEHVFRTFDANGDGTIDFREFIIALSVTSRGKLEQKLKWAFSMYDLDGNGYISKAEMLEIVQAIYKMVSSVMKMPEDESTPEKRTEKIFRQMDTNRDGKLSMEEFIRGAKSDPSIVRLLQCDPSSAGQF

相同性解析による交差性が予測される生物種
Full Name neurocalcin delta
Calculated molecular weight 193 aa, 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC036098
Gene Symbol NCALD
Gene ID (NCBI) 83988
RRIDAB_11232229
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP61601
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

NCALD(Neurocalcin-delta) is a member of the EF-hand calcium-binding protein superfamily.It may be involved in the calcium-dependent regulation of rhodopsin phosphorylation.It is a high-affinity Ca2+ sensor (Kd of approx. 0.6 uM), which is most abundantly expressed in the Purkinje cells of the cerebellum(PMID:11964161).This antibody is specific to NCALD.