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Validation Data Gallery

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  • Immunoprecipitation (IP) experiment of HeLa cells using NBPF1 Polyclonal antibody (13242-1-AP)

    IP experiment of HeLa using 13242-1-AP (same clone as 13242-1-PBS)

    IP result of anti-NBPF1 (IP:13242-1-AP, 4ug; Detection:13242-1-AP 1:300) with HeLa cells lysate 1760 ug. This data was developed using the same antibody clone with 13242-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using NBPF1 Polyclonal antibody (13242-1-AP)

    IHC staining of human liver using 13242-1-AP (same clone as 13242-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13242-1-AP (NBPF1 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13242-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using NBPF1 Polyclonal antibody (13242-1-AP)

    IF Staining of Jurkat using 13242-1-AP (same clone as 13242-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using NBPF1 antibody (13242-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 13242-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of BxPC-3 cells using NBPF1 Polyclonal antibody (13242-1-AP)

    IF Staining of BxPC-3 using 13242-1-AP (same clone as 13242-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed BxPC-3 cells using NBPF1 antibody (13242-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 13242-1-PBS in a different storage buffer formulation.

NBPF1 Polyclonal antibody, PBS Only

NBPF1 Polyclonal Antibody for IHC, IF/ICC, IP, Indirect ELISA
Cat No. 13242-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, IP, Indirect ELISA

Synonyms

KIAA1693, Neuroblastoma breakpoint family member 1

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13242-1-PBS targets NBPF1 in IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag4096

Product name: Recombinant human NBPF1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 303-603 aa of BC034418

Sequence: PSGYLELTDSCQPYRSAFYILEQQRVGWALDMDEIEKYQEVEEDQDPSCPRLSRELLDEKEPEVLQDSLDRCYSTPSGYLELPDLGQPYRSAVYSLEEQYLGLALDVDRIKKDQEEEEDQGPPCPRLSRELLEAVEPEVLQDSLDRCYSTPSSCLEQPDSCLPYGSSFYALEEKHVGFSLDVGEIEKKGKGKKRRGRRSTKKRRRRGRKEGEEDQNPPCPRLSGVLMEVEEPEVLQDSLDRCYSTPSMFFELPDSFQHYRSVFYSFEEQHISFALDVDNRFLTLMGRSLHLVFQMGVIFPQ

相同性解析による交差性が予測される生物種
Full Name neuroblastoma breakpoint family, member 1
Calculated molecular weight 1213 aa, 139 kDa
Observed molecular weight139 kDa
GenBank accession numberBC034418
Gene Symbol NBPF1
Gene ID (NCBI) 55672
RRIDAB_3669156
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ3BBV0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

NBPF1 (Neuroblastoma Breakpoint Family, member 1) was originally identified in a neuroblastoma patient on the basis of its disruption by a chromosomal translocation t(1;17)

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