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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NBEAL1 Polyclonal antibody (25431-1-AP)

    WB analysis using 25431-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25431-1-AP (NBEAL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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NBEAL1 Polyclonal antibody

NBEAL1 Polyclonal Antibody for WB, ELISA
Cat No. 25431-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

ALS2CR16, ALS2CR17, neurobeachin like 1, Neurobeachin like protein 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25431-1-AP targets NBEAL1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NBEAL1 fusion protein Ag22125 相同性解析による交差性が予測される生物種
Full Name neurobeachin-like 1
Calculated molecular weight 200 aa, 307 kDa
Observed molecular weight280 kDa
GenBank accession numberBC137214
Gene Symbol NBEAL1
Gene ID (NCBI) 65065
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6ZS30
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Neurobeachin-like 1 (NBEAL1) is a Golgi-associated protein that plays an essential role in cholesterol metabolism regulation.

Protocols

Product Specific Protocols
WB protocol for NBEAL1 antibody 25431-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols