Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, HL-60 cells, human brain tissue, HeLa cells, MG-63 cells, U2OS cells |
| Positive IP detected in | HL-60 cells |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
15807-1-AP targets NAT5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8541 Product name: Recombinant human NAT5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-178 aa of BC005181 Sequence: MTTLRAFTCDDLFRFNNINLDPLTETYGIPFYLQYLAHWPEYFIVAEAPGGELMGYIMGKAEGSVAREEWHGHVTALSVAPEFRRLGLAAKLMELLEEISERKGGFFVDLFVRVSNQVAVNMYKQLGYSVYRTVIEYYSASNGEPDEDAYDMRKALSRDTEKKSIIPLPHPVRPEDIE 相同性解析による交差性が予測される生物種 |
| Full Name | N-acetyltransferase 5 (GCN5-related, putative) |
| Calculated molecular weight | 178 aa, 20 kDa |
| Observed molecular weight | 18-20 kDa |
| GenBank accession number | BC005181 |
| Gene Symbol | NAT5 |
| Gene ID (NCBI) | 51126 |
| RRID | AB_1640056 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P61599 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
N-acetyltransferase 5 (NAT5/hNaa50p), also known as NAA20 (N-alpha-acetyltransferase 20), is a catalytic subunit of the N-terminal acetyltransferase B complex, which is a member of the GCN5-related N-acetyltransferase (GNAT) superfamily. It specifically acetylates the N-terminus of proteins (N-terminal acetylation) and is crucial for cell survival, chromosome segregation, and cell cycle progression. Naa20 expression is much higher in HCC tumors than in normal tissues, promoting oncogenic properties in HCC cells. Knockdown of NAA20 significantly inhibited TNBC cell viability by CCK8 and colony formation assays and cell migration and invasion (PMID: 37827343, 33219302).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for NAT5 antibody 15807-1-AP | Download protocol |
| IHC protocol for NAT5 antibody 15807-1-AP | Download protocol |
| IP protocol for NAT5 antibody 15807-1-AP | Download protocol |
| WB protocol for NAT5 antibody 15807-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int J Mol Sci NatB Catalytic Subunit Depletion Disrupts DNA Replication Initiation Leading to Senescence in MEFs | ||
Oncotarget NatB-mediated protein N-α-terminal acetylation is a potential therapeutic target in hepatocellular carcinoma. | ||
Mol Cell Biol NatB Protects Procaspase-8 from UBR4-Mediated Degradation and Is Required for Full Induction of the Extrinsic Apoptosis Pathway |