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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NAT2 Polyclonal antibody (11410-1-AP)

    WB analysis using 11410-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11410-1-AP (NAT2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using NAT2 Polyclonal antibody (11410-1-AP)

    IHC staining of human small intestine using 11410-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 11410-1-AP (NAT2 antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using NAT2 Polyclonal antibody (11410-1-AP)

    IHC staining of human breast cancer using 11410-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11410-1-AP (NAT2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using NAT2 Polyclonal antibody (11410-1-AP)

    IHC staining of human breast cancer using 11410-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11410-1-AP (NAT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using NAT2 Polyclonal antibody (11410-1-AP)

    IHC staining of human breast cancer using 11410-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11410-1-AP (NAT2 Antibody) at dilution of 1:50 (under 40x lens).

  • Flow cytometry (FC) experiment of MCF-7 cells using NAT2 Polyclonal antibody (11410-1-AP)

    FC experiment of MCF-7 using 11410-1-AP

    1x10^6 MCF-7 cells were intracellularly stained with 0.8 ug NAT2 Polyclonal antibody (11410-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

NAT2 Polyclonal antibody

NAT2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11410-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

AAC2, Arylamide acetylase 2, Arylamine N-acetyltransferase 2, EC:2.3.1.118, EC:2.3.1.5

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inrat liver tissue
Positive IHC detected inhuman small intestine tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11410-1-AP targets NAT2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1953

Product name: Recombinant human NAT2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-290 aa of BC015878

Sequence: MDIEAYFERIGYKNSRNKLDLETLTDILEHQIRAVPFENLNMHCGQAMELGLEAIFDHIVRRNRGGWCLQVNQLLYWALTTIGFQTTMLGGYFYIPPVNKYSTGMVHLLLQVTIDGRNYIVDAGSGSSSQMWQPLELISGKDQPQVPCIFCLTEERGIWYLDQIRREQYITNKEFLNSHLLPKKKHQKIYLFTLEPQTIEDFESMNTYLQTSPTSSFITTSFCSLQTPEGVYCLVGFILTYRKFNYKDNTDLVEFKTLTEEEVEEVLKNIFKISLGRNLVPKPGDGSLTI

相同性解析による交差性が予測される生物種
Full Name N-acetyltransferase 2 (arylamine N-acetyltransferase)
Calculated molecular weight 33.5 kDa
Observed molecular weight38 kDa
GenBank accession numberBC015878
Gene Symbol NAT2
Gene ID (NCBI) 10
RRIDAB_3085371
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP11245
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NAT2 (Arylamine N-acetyltransferase 2) is well-known for its role in the phase II metabolism of xenobiotics and drugs (PMID:36156098). NAT2 and its isozyme, arylamine N-acetyltransferase 1 (NAT1), play key roles in the detoxification of carcinogenic arylamines and xenobiotics by catalyzing acetyl-coenzyme A (CoA)-dependent biotransformation of arylamines to N-arylacetamides. Endogenous NAT2 expression is limited to the liver and small and large intestines, suggesting that NAT2 plays organ-specific roles (PMID:24467436).

Protocols

Product Specific Protocols
IHC protocol for NAT2 antibody 11410-1-APDownload protocol
WB protocol for NAT2 antibody 11410-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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