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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NAT12 Polyclonal antibody (25149-1-AP)

    WB analysis using 25149-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25149-1-AP (NAT12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using NAT12 Polyclonal antibody (25149-1-AP)

    IF Staining of U2OS using 25149-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using NAT12 antibody (25149-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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NAT12 Polyclonal antibody

NAT12 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 25149-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

C14orf35, MAK3, Mak3p, N acetyltransferase 12, NAA30, NAT12

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25149-1-AP targets NAT12 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NAT12 fusion protein Ag18435 相同性解析による交差性が予測される生物種
Full Name N-acetyltransferase 12 (GCN5-related, putative)
Calculated molecular weight 362 aa, 39 kDa
Observed molecular weight40 kDa
GenBank accession numberBC118589
Gene Symbol NAT12
Gene ID (NCBI) 122830
RRIDAB_3085773
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ147X3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

N-terminal acetylation (Nt-acetylation) by N-terminal acetyltransferases (NATs) is one of the most common protein modifications in eukaryotes. The NatC complex represents one of three major NATs of which the substrate profile remains largely unexplored. NAT12/NAA30 (N-alpha-acetyltransferase 30) is the catalytic subunit of the NAT-C complex (PMID: 27694331, 26292663). The expected sizes of the two isoforms of the protein were 35 and 39 kDa. Western blots detected two bands of NAA12, the expected ~40 kDa band and a possibly dimer at ~70 kDa.

Protocols

Product Specific Protocols
WB protocol for NAT12 antibody 25149-1-APDownload protocol
IF protocol for NAT12 antibody 25149-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols