Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells, mouse spleen tissue |
| Positive IHC detected in | human ovarian cancer Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IF | See 1 publications below |
Product Information
16698-1-AP targets NAA40 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | NAA40 fusion protein Ag10092 相同性解析による交差性が予測される生物種 |
| Full Name | N-acetyltransferase 11 (GCN5-related, putative) |
| Calculated molecular weight | 237 aa, 27 kDa |
| Observed molecular weight | 27 kDa |
| GenBank accession number | BC052298 |
| Gene Symbol | NAA40 |
| Gene ID (NCBI) | 79829 |
| RRID | AB_3085505 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86UY6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
N-alpha-acetyltransferase 40 (NAA40, also known as NatD, Nat4, or Patt1) is a highly specific epigenetic enzyme catalyzing the transfer of an acetyl moiety at the N-terminal end of histones H4 and H2A (PMID: 26666750). NAA40 is significantly upregulated in primary CRC tissues and promotes CRC cell growth both in vitro and in xenograft tumor models (PMID: 30858358). Western blot analysis detected NAA40 at an apparent molecular mass of 27 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NAA40 antibody 16698-1-AP | Download protocol |
| IHC protocol for NAA40 antibody 16698-1-AP | Download protocol |
| IF protocol for NAA40 antibody 16698-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Biol Toxicol RNF112, whose transcription is regulated by KLF4, inhibits colorectal cancer growth via promoting ubiquitin-dependent degradation of NAA40 |