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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NASP Monoclonal antibody

NASP Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1A1A1

Cat no : 68710-1-Ig

Synonyms

DKFZp547F162, FLB7527, FLJ31599, FLJ35510, NASP, PRO1999

Validation Data Gallery

Applications:
  • WB analysis using 68710-1-Ig

    WB analysis using 68710-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68710-1-Ig (NASP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, Ramos cells, U2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Sample-dependent, check data in validation data gallery

Product Information

68710-1-Ig targets NASP in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NASP fusion protein Ag1824 相同性解析による交差性が予測される生物種
Full Name nuclear autoantigenic sperm protein (histone-binding)
Calculated molecular weight 788 aa, 85 kDa
Observed molecular weight150 kDa, 70 kDa
GenBank accession numberBC010105
Gene symbol NASP
Gene ID (NCBI) 4678
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NASP (nuclear autoantigenic sperm protein) is associated with DNA replication, cell proliferation and cell cycle progression through functioning as a Histone H1 binding protein that mediates histone transport to the nucleus. NASP had two isoforms, tNASP (testis type) and sNASP (somatic type). The tNASP was mainly expressed in testis, a variety of malignant tumors, stem cells and embryonic tissues, while sNASP existed in all somatic mitosis cells. This antibody can recognize both isoforms.

Protocols

Product Specific Protocols
WB protocol for NASP antibody 68710-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols