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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using NARG1L Polyclonal antibody (17427-1-AP)

    WB analysis of Jurkat using 17427-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 17427-1-AP (NARG1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using NARG1L Polyclonal antibody (17427-1-AP)

    WB analysis of Jurkat using 17427-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 17427-1-AP (NARG1L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using NARG1L Polyclonal antibody (17427-1-AP)

    IF Staining of HEK-293 using 17427-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using NARG1L antibody (17427-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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NARG1L Polyclonal antibody

NARG1L Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 17427-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

NMDA receptor-regulated 1-like protein, NAT2, NARG1-like protein, NARG1 like protein, N-alpha-acetyltransferase 16, NatA auxiliary subunit

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17427-1-AP targets NARG1L in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NARG1L fusion protein Ag11492 相同性解析による交差性が予測される生物種
Full Name NMDA receptor regulated 1-like
Calculated molecular weight 864 aa, 101 kDa
Observed molecular weight 97-100 kDa
GenBank accession numberBC028112
Gene Symbol NARG1L
Gene ID (NCBI) 79612
RRIDAB_1939557
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6N069
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NMDA receptor-regulated 1-like protein (NARG1L, also known as NAA16), is the auxillary subunit of the N-terminal acetyltransferase A (NatA) complex which displays alpha (N-terminal) acetyltransferase activity.

Protocols

Product Specific Protocols
WB protocol for NARG1L antibody 17427-1-APDownload protocol
IF protocol for NARG1L antibody 17427-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols