• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NAPG Polyclonal antibody

NAPG Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 15604-1-AP

Synonyms

GAMMASNAP, NAPG, SNAP gamma, SNAPG

Validation Data Gallery

Applications:
  • WB analysis using 15604-1-AP

    WB analysis using 15604-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15604-1-AP (NAPG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 15604-1-AP

    WB analysis of HeLa using 15604-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15604-1-AP (NAPG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 15604-1-AP

    WB analysis of HepG2 using 15604-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15604-1-AP (NAPG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of K-562 using 15604-1-AP

    WB analysis of K-562 using 15604-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 15604-1-AP (NAPG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 15604-1-AP

    IP experiment of HepG2 using 15604-1-AP

    IP result of anti-NAPG (IP:15604-1-AP, 4ug; Detection:15604-1-AP 1:400) with HepG2 cells lysate 3300 ug.

Tested Applications

Positive WB detected inA375 cells, K-562 cells, HepG2 cells, HeLa cells, Jurkat cells
Positive IP detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Sample-dependent, check data in validation data gallery

Product Information

15604-1-AP targets NAPG in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NAPG fusion protein Ag7988 相同性解析による交差性が予測される生物種
Full Name N-ethylmaleimide-sensitive factor attachment protein, gamma
Calculated molecular weight 35 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC001889
Gene symbol NAPG
Gene ID (NCBI) 8774
RRIDAB_2150885
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NAPG antibody 15604-1-APDownload protocol
IP protocol for NAPG antibody 15604-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols