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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NAPB Polyclonal antibody (14106-1-AP)

    WB analysis using 14106-1-AP (same clone as 14106-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14106-1-AP (NAPB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14106-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using NAPB Polyclonal antibody (14106-1-AP)

    WB analysis of mouse brain using 14106-1-AP (same clone as 14106-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14106-1-AP (NAPB antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14106-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using NAPB Polyclonal antibody (14106-1-AP)

    IP experiment of mouse brain using 14106-1-AP (same clone as 14106-1-PBS)

    IP result of anti-NAPB (IP:14106-1-AP, 4ug; Detection:14106-1-AP 1:1000) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 14106-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using NAPB Polyclonal antibody (14106-1-AP)

    IHC staining of mouse brain using 14106-1-AP (same clone as 14106-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14106-1-AP (NAPB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14106-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using NAPB Polyclonal antibody (14106-1-AP)

    IHC staining of human gliomas using 14106-1-AP (same clone as 14106-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14106-1-AP (NAPB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14106-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using NAPB Polyclonal antibody (14106-1-AP)

    IHC staining of human gliomas using 14106-1-AP (same clone as 14106-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14106-1-AP (NAPB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14106-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NAPB Polyclonal antibody (14106-1-AP)

    IF Staining of HepG2 using 14106-1-AP (same clone as 14106-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using NAPB antibody 14106-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 14106-1-PBS in a different storage buffer formulation.

NAPB Polyclonal antibody, PBS Only

NAPB Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 14106-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14106-1-PBS targets NAPB in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag5239

Product name: Recombinant human NAPB protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-298 aa of BC060840

Sequence: MDNAGKEREAVQLMAEAEKRVKASHSFLRGLFGGNTRIEEACEMYTRAANMFKMAKNWSAAGNAFCQAAKLHMQLQSKHDSATSFVDAGNAYKKADPQEAINCLNAAIDIYTDMGRFTIAAKHHITIAEIYETELVDIEKAIAHYEQSADYYKGEESNSSANKCLLKVAAYAAQLEQYQKAIEIYEQVGANTMDNPLLKYSAKDYFFKAALCHFIVDELNAKLALEKYEEMFPAFTDSRECKLLKKLLEAHEEQNSEAYTEAVKEFDSISRLDQWLTTMLLRIKKSIQGDGEGDGDLK

相同性解析による交差性が予測される生物種
Full Name N-ethylmaleimide-sensitive factor attachment protein, beta
Calculated molecular weight 298 aa, 34 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC060840
Gene Symbol NAPB
Gene ID (NCBI) 63908
RRIDAB_2185010
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H115
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.