Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HuH-7 cells, mouse heart tissue, mouse testis tissue, rat heart tissue, U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
27889-1-AP targets NAP1L4 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.
| Tested Reactivity | Human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag27469 Product name: Recombinant human NAP1L4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 222-375 aa of BC022090 Sequence: TKTYKMKSEPDKADPFSFEGPEIVDCDGCTIDWKKGKNVTVKTIKKKQKHKGRGTVRTITKQVPNESFFNFFNPLKASGDGESLDEDSEFTLASDFEIGHFFRERIVPRAVLYFTGEAIEDDDNFEEGEEGEEEELEGDEEGEDEDDAEINPKV 相同性解析による交差性が予測される生物種 |
| Full Name | nucleosome assembly protein 1-like 4 |
| Calculated molecular weight | 375 aa, 43 kDa |
| Observed molecular weight | 52 kDa |
| GenBank accession number | BC022090 |
| Gene Symbol | NAP1L4 |
| Gene ID (NCBI) | 4676 |
| RRID | AB_2881006 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q99733 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NAP1L4 is a member of the nucleosome assembly protein (NAP) family which can interact with both core and linker histones. It can shuttle between the cytoplasm and nucleus, suggesting a role as a histone chaperone. This gene is one of several located near the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NAP1L4 antibody 27889-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
