Validation Data Gallery

Filter: AllWBIPIHC

WB analysis of HeLa using 16018-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 16018-1-AP (NAP1L4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 16018-1-AP
WB result of NAP1L4 antibody (16018-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NAP1L4 transfected HeLa cells.
WB analysis of HEK-293 using 16018-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 16018-1-AP (NAP1L4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 16018-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 16018-1-AP (NAP1L4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 16018-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 16018-1-AP (NAP1L4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 16018-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 16018-1-AP (NAP1L4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 16018-1-AP
IP result of anti-NAP1L4 (IP:16018-1-AP, 4ug; Detection:16018-1-AP 1:1000) with HeLa cells lysate 4000ug.
IHC staining of human liver using 16018-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 16018-1-AP (NAP1L4 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human liver using 16018-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 16018-1-AP (NAP1L4 antibody) at dilution of 1:100 (under 40x lens).

Featured Product
KD/KO Validated

NAP1L4 Polyclonal antibody

NAP1L4 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 16018-1-AP

Publications(8)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, RIP, ELISA

Synonyms

hNAP2, NAP 2, NAP1L4, NAP2, NAP2L, Nucleosome assembly protein 2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (8)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, mouse liver tissue, mouse testis tissue, HEK-293 cells, A431 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 7 publications below
IHC	See 1 publications below
IF	See 2 publications below
RIP	See 1 publications below

Product Information

16018-1-AP targets NAP1L4 in WB, IHC, IF, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag8827 Product name: Recombinant human NAP1L4 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-375 aa of BC022090 Sequence: MADHSFSDGVPSDSVEAAKNASNTEKLTDQVMQNPRVLAALQERLDNVPHTPSSYIETLPKAVKRRINALKQLQVRCAHIEAKFYEEVHDLERKYAALYQPLFDKRREFITGDVEPTDAESEWHSENEEEEKLAGDMKSKVVVTEKAAATAEEPDPKGIPEFWFTIFRNVDMLSELVQEYDEPILKHLQDIKVKFSDPGQPMSFVLEFHFEPNDYFTNSVLTKTYKMKSEPDKADPFSFEGPEIVDCDGCTIDWKKGKNVTVKTIKKKQKHKGRGTVRTITKQVPNESFFNFFNPLKASGDGESLDEDSEFTLASDFEIGHFFRERIVPRAVLYFTGEAIEDDDNFEEGEEGEEEELEGDEEGEDEDDAEINPKV 相同性解析による交差性が予測される生物種
Full Name	nucleosome assembly protein 1-like 4
Calculated molecular weight	375 aa, 43 kDa
Observed molecular weight	52 kDa
GenBank accession number	BC022090
Gene Symbol	NAP1L4
Gene ID (NCBI)	4676
RRID	AB_2150711
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q99733
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IHC protocol for NAP1L4 antibody 16018-1-AP	Download protocol
IP protocol for NAP1L4 antibody 16018-1-AP	Download protocol
WB protocol for NAP1L4 antibody 16018-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	J Cell Biol Phase separation and toxicity of C9orf72 poly(PR) depends on alternate distribution of arginine. Authors - Chen Chen View Article
human	WB	Mol Cell Proteomics A novel immuno-competitive capture mass spectrometry strategy for protein-protein interaction profiling reveals that LATS kinases regulate HCV replication through NS5A phosphorylation. Authors - Hélène Meistermann View Article
mouse	IF,WB	J Virol NAP1L1 and NAP1L4 binding to Hypervariable Domain of Chikungunya Virus nsP3 Protein is bivalent and requires phosphorylation. Authors - Francisco Dominguez View Article
human	WB,IHC	J Dermatol Sci Nucleosome assembly protein 1-like 4, a new therapeutic target for proliferation and invasion of melanoma cells. Authors - Satoru Mizuhashi KD Validated View Article
human	WB	Nat Struct Mol Biol Mechanism of client selection by the protein quality-control factor UBE2O Authors - Matthew C J Yip View Article
human	WB	iScience Differential toxicity and localization of arginine-rich C9ORF72 dipeptide repeat proteins depend on de-clustering of positive charges Authors - Tamami Miyagi View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa using 16018-1-AP

WB analysis of HeLa using 16018-1-AP

WB analysis of HEK-293 using 16018-1-AP

WB analysis of A431 using 16018-1-AP

WB analysis of mouse liver using 16018-1-AP

WB analysis of mouse testis using 16018-1-AP

IP experiment of HeLa using 16018-1-AP

IHC staining of human liver using 16018-1-AP

IHC staining of human liver using 16018-1-AP

NAP1L4 Polyclonal antibody

NAP1L4 Polyclonal Antibody for WB, IP, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Phase separation and toxicity of C9orf72 poly(PR) depends on alternate distribution of arginine.

A novel immuno-competitive capture mass spectrometry strategy for protein-protein interaction profiling reveals that LATS kinases regulate HCV replication through NS5A phosphorylation.

NAP1L1 and NAP1L4 binding to Hypervariable Domain of Chikungunya Virus nsP3 Protein is bivalent and requires phosphorylation.

Nucleosome assembly protein 1-like 4, a new therapeutic target for proliferation and invasion of melanoma cells.

Mechanism of client selection by the protein quality-control factor UBE2O

Differential toxicity and localization of arginine-rich C9ORF72 dipeptide repeat proteins depend on de-clustering of positive charges