Validation Data Gallery
Tested Applications
| Positive WB detected in | K-562 cells, Neuro-2a cells, mouse brain tissue |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12519-1-AP targets NANP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3212 Product name: Recombinant human NANP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-248 aa of BC022552 Sequence: MGLSRVRAVFFDLDNTLIDTAGASRRGMLEVIKLLQSKYHYKEEAEIICDKVQVKLSKECFHPYNTCITDLRTSHWEEAIQETKGGAANRKLAEECYFLWKSTRLQHMTLAEDVKAMLTELRKEVRLLLLTNGDRQTQREKIEACACQSYFDAVVVGGEQREEKPAPSIFYYCCNLLGVQPGDCVMVGDTLETDIQGGLNAGLKATVWINKNGIVPLKSSPVPHYMVSSVLELPALLQSIDCKVSMST 相同性解析による交差性が予測される生物種 |
| Full Name | N-acetylneuraminic acid phosphatase |
| Calculated molecular weight | 248 aa, 28 kDa |
| Observed molecular weight | 29 kDa |
| GenBank accession number | BC022552 |
| Gene Symbol | NANP |
| Gene ID (NCBI) | 140838 |
| RRID | AB_3085396 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8TBE9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NANP, also known as HDHD4, C20orf147, is a member of the haloacid dehalogenase family of proteins. NANP enables N-acylneuraminate-9-phosphatase activity and is involved in N-acetylneuraminate biosynthetic process.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for NANP antibody 12519-1-AP | Download protocol |
| IHC protocol for NANP antibody 12519-1-AP | Download protocol |
| WB protocol for NANP antibody 12519-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
