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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using NANOS3 Polyclonal antibody (21679-1-AP)

    WB analysis of mouse testis using 21679-1-AP (same clone as 21679-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 21679-1-AP (NANOS3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21679-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using NANOS3 Polyclonal antibody (21679-1-AP)

    WB analysis of HEK-293 using 21679-1-AP (same clone as 21679-1-PBS)

    WB result of NANOS3 antibody (21679-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-NANOS3 transfected HEK-293 cells. This data was developed using the same antibody clone with 21679-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using NANOS3 Polyclonal antibody (21679-1-AP)

    IHC staining of human testis using 21679-1-AP (same clone as 21679-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 21679-1-AP (NANOS3 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 21679-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using NANOS3 Polyclonal antibody (21679-1-AP)

    IHC staining of human testis using 21679-1-AP (same clone as 21679-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 21679-1-AP (NANOS3 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 21679-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

NANOS3 Polyclonal antibody, PBS Only

NANOS3 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 21679-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

NOS-3, NOS3, NOS 3, NANOS1L, nanos homolog 3 (Drosophila)

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21679-1-PBS targets NANOS3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag16014

Product name: Recombinant human NANOS3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-192 aa of BC101209

Sequence: MGTFDLWTDYLGLAHLVRALSGKEGPETRLSPQPEPEPMLEPVSALEPMPAPESVPVPGPKDQKRSLESSPAPERLCSFCKHNGESRAIYQSHVLKDEAGRVLCPILRDYVCPQCGATRERAHTRRFCPLTGQGYTSVYSHTTRNSAGKKLVRPDKAKTQDTGHRRGGGGGAGFRGAGKSEPSPSCSPSMST

相同性解析による交差性が予測される生物種
Full Name nanos homolog 3 (Drosophila)
Calculated molecular weight 192 aa, 21 kDa
Observed molecular weight 26-30 kDa
GenBank accession numberBC101209
Gene Symbol NANOS3
Gene ID (NCBI) 342977
RRIDAB_10859250
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP60323
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The NANOS gene family is required for germ cell development in diverse organisms. NANOS3 is found in migrating primordial germ cells, and the homozygous deficiency of NANOS3 in the mouse results in the complete loss of germ cells in both sexes.

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