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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Nanog Recombinant antibody (80210-13-RR)

    WB analysis using 80210-13-RR (same clone as 80210-13-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80210-13-RR (NANOG antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80210-13-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Nanog Recombinant antibody (80210-13-RR)

    WB analysis using 80210-13-RR (same clone as 80210-13-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80210-13-RR (NANOG antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80210-13-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP02157-2

    Sandwich ELISA standard curve of MP02157-2

    Sandwich ELISA standard curve of MP02157-2, Human Nanog Recombinant Matched Antibody Pair - PBS only. 80210-13-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag5645. 80210-12-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

  • Sample test of MP02157-2

    Sample test of MP02157-2

    The mean Nanog concentration was determined to be 9.25 ng/mL in NCCIT cell extract based on a 1.50 mg/mL extract load.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Nanog Recombinant antibody (80210-13-RR)

    IHC staining of mouse brain using 80210-13-RR (same clone as 80210-13-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 80210-13-RR (Nanog antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80210-13-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse embryo tissue using Nanog Recombinant antibody (80210-13-RR)

    IHC staining of mouse embryo using 80210-13-RR (same clone as 80210-13-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 80210-13-RR (Nanog antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80210-13-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse embryo tissue using Nanog Recombinant antibody (80210-13-RR)

    IHC staining of mouse embryo using 80210-13-RR (same clone as 80210-13-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 80210-13-RR (Nanog antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80210-13-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Nanog Recombinant antibody (80210-13-RR)

    IHC staining of mouse brain using 80210-13-RR (same clone as 80210-13-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 80210-13-RR (Nanog antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80210-13-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NCCIT cells using Nanog Recombinant antibody (80210-13-RR)

    IF Staining of NCCIT using 80210-13-RR (same clone as 80210-13-PBS)

    Immunofluorescent analysis of (4% PFA) fixed NCCIT cells using Nanog antibody (80210-13-RR, Clone: 250067F10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80210-13-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP02157-2

    Cytometric bead array standard curve of MP02157-2

    Cytometric bead array standard curve of MP02157-2, Nanog Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 80210-13-PBS. Detection antibody: 80210-12-PBS. Standard: Ag5645. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 80210-13-RR

    Affinity and Kinetic Characterization of 80210-13-RR

    Biolayer interferometry (BLl) kinetic assays of 80210-13-RR against Human Nanog were performed. The affinity constant is 11.0 nM.

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Nanog Recombinant antibody, PBS Only (Capture)

Nanog Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 80210-13-PBS
Clone No.250067F10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

hNanog

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80210-13-PBS targets Nanog as part of a matched antibody pair:

MP02157-1: 80210-11-PBS capture and 80210-13-PBS detection (validated in Cytometric bead array)

MP02157-2: 80210-13-PBS capture and 80210-12-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Nanog fusion protein Ag5645 相同性解析による交差性が予測される生物種
Full Name Nanog homeobox
Calculated molecular weight 35 kDa
Observed molecular weight35-40 kDa
GenBank accession numberBC160187
Gene Symbol Nanog
Gene ID (NCBI) 79923
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9H9S0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Nanog is a member of the homeobox family of DNA binding transcription factors and has been shown to maintain embryonic stem (ES) cell self-renewal independently of leukemia inhibitory factor (LIF)/Stat3. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Functionally, Nanog works together with other key pluripotent factors (Oct4, Sox2, and Lin28) to reprogram human fibroblasts and generate induced pluripotent stem (iPS) cells. These key factors form a regulatory network to support or limit each other's expression level, which maintains the properties of ES cells. There are two kinds of variants that can be recognized by NANOG, one is a normal form (~39 kDa), the other is a post-translation modified form (~48 kDa) (21136380 ). Nanog has two isoforms with molecular weights of 34.4 kDa and 31.9 kDa. (PMID: 21969378)