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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NAGS Polyclonal antibody (21566-1-AP)

    WB analysis using 21566-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21566-1-AP (NAGS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using NAGS Polyclonal antibody (21566-1-AP)

    WB analysis of HepG2 using 21566-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 21566-1-AP (NAGS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using NAGS Polyclonal antibody (21566-1-AP)

    WB analysis of L02 using 21566-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 21566-1-AP (NAGS antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using NAGS Polyclonal antibody (21566-1-AP)

    WB analysis of Jurkat using 21566-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 21566-1-AP (NAGS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using NAGS Polyclonal antibody (21566-1-AP)

    IHC staining of human liver using 21566-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 21566-1-AP (NAGS antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using NAGS Polyclonal antibody (21566-1-AP)

    IHC staining of human liver using 21566-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 21566-1-AP (NAGS antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using NAGS Polyclonal antibody (21566-1-AP)

    IHC staining of human small intestine using 21566-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine using 21566-1-AP (NAGS antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using NAGS Polyclonal antibody (21566-1-AP)

    IHC staining of human small intestine using 21566-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine using 21566-1-AP (NAGS antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NAGS Polyclonal antibody (21566-1-AP)

    IF Staining of HepG2 using 21566-1-AP

    Immunofluorescent analysis of HepG2 cells, using NAGS antibody 21566-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NAGS Polyclonal antibody (21566-1-AP)

    IF Staining of HeLa using 21566-1-AP

    Immunofluorescent analysis of Hela cells, using NAGS antibody 21566-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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NAGS Polyclonal antibody

NAGS Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 21566-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

AGAS, Amino acid acetyltransferase, ARGA, N acetylglutamate synthase, NAGS

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse liver tissue, Jurkat cells, HepG2 cells, L02 cells, rat liver tissue
Positive IHC detected inhuman liver tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, Hela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21566-1-AP targets NAGS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NAGS fusion protein Ag16073 相同性解析による交差性が予測される生物種
Full Name N-acetylglutamate synthase
Calculated molecular weight 534 aa, 58 kDa
Observed molecular weight 58-66 kDa
GenBank accession numberBC111713
Gene Symbol NAGS
Gene ID (NCBI) 162417
RRIDAB_10733886
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N159
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

N-acetylglutamate synthase, also known as NAGS, is a mitochondrial enzyme that catalyzes the formation of N-acetylglutamate (NAG) from glutamate and acetyl coenzyme-A. NAGS is the key enzyme for regulating the hepatic urea cycle and is also highly expressed in the kidney and gut. Deficiency of NAGS leads to hyperammonemia due to decreased activity of CPSI deprived of its cofactor NAG(PMID:12459178,12754705).

Protocols

Product Specific Protocols
WB protocol for NAGS antibody 21566-1-APDownload protocol
IHC protocol for NAGS antibody 21566-1-APDownload protocol
IF protocol for NAGS antibody 21566-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Metab

SWI/SNF complex subunit BAF60a represses hepatic ureagenesis through a crosstalk between YB-1 and PGC-1α.

Authors - Wenxiang Zhang
View Article
human,mouseWB,IHC

Heliyon

Machine learning deciphers the significance of mitochondrial regulators on the diagnosis and subtype classification in non-alcoholic fatty liver disease

Authors - Bingyu Wang
View Article