Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, L02 cells, U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31365-1-AP targets NAGPA in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag34860 Product name: Recombinant human NAGPA protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 50-309 aa of BC012194 Sequence: DCTRVRAGNREHESWPPPPATPGAGGLAVRTFVSHFRDRAVAGHLTRAVEPLRTFSVLEPGGPGGCAARRRATVEETARAADCRVAQNGGFFRMNSGECLGNVVSDERRVSSSGGLQNAQFGIRRDGTLVTGYLSEEEVLDTENPFVQLLSGVVWLIRNGSIYINESQATECDETQETGSFSKFVNVISARTAIGHDRKGQLVLFHADGQTEQRGINLWEMAEFLLKQDVVNAINLDGGGSATFVLNGTLASYPSDHWQA 相同性解析による交差性が予測される生物種 |
| Full Name | N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase |
| Calculated molecular weight | 515aa,56 kDa; 309aa,34 kDa |
| Observed molecular weight | 60 kDa |
| GenBank accession number | BC012194 |
| Gene Symbol | NAGPA |
| Gene ID (NCBI) | 51172 |
| RRID | AB_3742419 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q9UK23 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NAGPA antibody 31365-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |