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Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using NAF1 Polyclonal antibody (27764-1-AP)

    WB analysis of K-562 using 27764-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 27764-1-AP (NAF1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using NAF1 Polyclonal antibody (27764-1-AP)

    WB analysis of HL-60 using 27764-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 27764-1-AP (NAF1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using NAF1 Polyclonal antibody (27764-1-AP)

    IHC staining of human ovary cancer using 27764-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27764-1-AP (NAF1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using NAF1 Polyclonal antibody (27764-1-AP)

    IHC staining of human ovary cancer using 27764-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27764-1-AP (NAF1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using NAF1 Polyclonal antibody (27764-1-AP)

    IHC staining of human ovary cancer using 27764-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27764-1-AP (NAF1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

NAF1 Polyclonal antibody

NAF1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27764-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

hNAF1, H/ACA ribonucleoprotein complex non-core subunit NAF1

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inK-562 cells, HL-60 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27764-1-AP targets NAF1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag26787

Product name: Recombinant human NAF1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-345 aa of BC008207

Sequence: MEVVEAAAAQLETLKFNGTDFGVGEGPAAPSPGSAPVPGTQPPLQSFEGSPDAGQTVEVKPAGEQPLQPVLNAVAAGTPAPQPQPPAESPACGDCVTSPGAAEPARAPDSLETSDSDSDSDSETDSDSSSSSSSSSSSSSSSSSSCISLPPVLSDGDDDLQVEKENKNFPLKTKDELLLNELPSVEELTIILPEDIELKPLGMVSSIIEQLVIIESMTNLPPVNEETVIFKSDRQAAGKIFEIFGPVAHPFYVLRFNSSDHIESKGIKIKETMYFAPSMKDFTQYIFTEKLKQDKGSDASWKNDQEPPPEALDFSDDEKEKEAKQRKKSQIQGRKKLKSEFNEPG

相同性解析による交差性が予測される生物種
Full Name nuclear assembly factor 1 homolog (S. cerevisiae)
Calculated molecular weight 54 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC008207
Gene Symbol NAF1
Gene ID (NCBI) 92345
RRIDAB_2880965
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96HR8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NAF1, an H/ACA box RNP assembly chaperone, is a factor necessary for H/ACA snoRNP maturation and ribosome biosynthesis in mammalian cells. In addition, among these RNA biogenesis factors, NAF1 may be unique in that it is required at full dosage for telomerase and telomere maintenance in the process of pulmonary fibrosis-emphysema. (PMID: 30936423)

Protocols

Product Specific Protocols
IHC protocol for NAF1 antibody 27764-1-APDownload protocol
WB protocol for NAF1 antibody 27764-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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