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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NADK Polyclonal antibody (29290-1-AP)

    WB analysis using 29290-1-AP (same clone as 29290-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29290-1-AP (NADK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29290-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using NADK Polyclonal antibody (29290-1-AP)

    WB analysis of HEK-293 using 29290-1-AP (same clone as 29290-1-PBS)

    WB result of NADK antibody (29290-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-NADK transfected HEK-293 cells. This data was developed using the same antibody clone with 29290-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using NADK Polyclonal antibody (29290-1-AP)

    IHC staining of human stomach cancer using 29290-1-AP (same clone as 29290-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 29290-1-AP (NADK antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29290-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using NADK Polyclonal antibody (29290-1-AP)

    IHC staining of human ovary cancer using 29290-1-AP (same clone as 29290-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 29290-1-AP (NADK antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29290-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

NADK Polyclonal antibody, PBS Only

NADK Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 29290-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

EC:2.7.1.23, NAD kinase, Poly(P)/ATP NAD kinase

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29290-1-PBS targets NADK in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag30982

Product name: Recombinant human NADK protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 238-446 aa of BC001709

Sequence: SRLKVRVVKELRGKKTAVHNGLGEKGSQAAGLDMDVGKQAMQYQVLNEVVIDRGPSSYLSNVDVYLDGHLITTVQGDGVIVSTPTGSTAYAAAAGASMIHPNVPAIMITPICPHSLSFRPIVVPAGVELKIMLSPEARNTAWVSFDGRKRQEIRHGDSISITTSCYPLPSICVRDPVSDWFESLAQCLHWNVRKKQAHFEEEEEEEEEG

相同性解析による交差性が予測される生物種
Full Name NAD kinase
Calculated molecular weight 49 kDa
Observed molecular weight 49 kDa
GenBank accession numberBC001709
Gene Symbol NADK
Gene ID (NCBI) 65220
RRIDAB_2918272
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO95544
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

NAD kinase (NADK) is the sole NADP+-biosynthetic enzyme that catalyzes phosphorylation of NAD+ to yield NADP+ using ATP as a phosphoryl donor, and thus, plays a vital role in the cell and represents a potentially powerful antimicrobial drug target (PMID:21526340). It belongs to the NAD kinase family. NADK has 3 isoforms with the molecular mass of 49, 63 and 46 kDa. The catalytically active human NADK is a homotetramer (PMID:11594753).

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