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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NAC1 Polyclonal antibody (14551-1-AP)

    WB analysis using 14551-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14551-1-AP (NAC1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using NAC1 Polyclonal antibody (14551-1-AP)

    WB analysis of MCF-7 using 14551-1-AP

    WB result of NAC1 antibody (14551-1-AP; 1:800; incubated at room temperature for 1.5 hours) with sh-Control and sh-NAC1 transfected MCF-7 cells.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using NAC1 Polyclonal antibody (14551-1-AP)

    IP experiment of MCF-7 using 14551-1-AP

    IP result of anti-NAC1 (IP:14551-1-AP, 4ug; Detection:14551-1-AP 1:300) with MCF-7 cells lysate 3200ug.

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using NAC1 Polyclonal antibody (14551-1-AP)

    IHC staining of mouse ovary using 14551-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 14551-1-AP (NAC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using NAC1 Polyclonal antibody (14551-1-AP)

    IHC staining of mouse ovary using 14551-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 14551-1-AP (NAC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using NAC1 Polyclonal antibody (14551-1-AP)

    IHC staining of human ovary tumor using 14551-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14551-1-AP (NAC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using NAC1 Polyclonal antibody (14551-1-AP)

    IHC staining of human ovary tumor using 14551-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14551-1-AP (NAC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using NAC1 Polyclonal antibody (14551-1-AP)

    IHC staining of mouse ovary using 14551-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 14551-1-AP (NAC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using NAC1 Polyclonal antibody (14551-1-AP)

    IF Staining of MCF-7 using 14551-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using NAC1 antibody (14551-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • KD/KO Validated

NAC1 Polyclonal antibody

NAC1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 14551-1-AP
Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

NACC1, BEND8, BTB/POZ domain-containing protein 14B, BTBD14B, NAC-1

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inLNCaP cells, MCF-7 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inmouse ovary tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

14551-1-AP targets NAC1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag6096

Product name: Recombinant human NACC1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 173-527 aa of BC055396

Sequence: SDSVQCMPVAKRLWDSGQKEAGGGGNGSRKMAKFSTPDLAANRPHQPPPPQQAPVVAAAQPAVAAGAGQPAGGVAAAGGVVSGPSTSERTSPGTSSAYTSDSPGSYHNEEDEEEDGGEEGMDEQYRQICNMYTMYSMMNVGQTAEKVEALPEQVAPESRNRIRVRQDLASLPAELINQIGNRCHPKLYDEGDPSEKLELVTGTNVYITRAQLMNCHVSAGTRHKVLLRRLLASFFDRNTLANSCGTGIRSSTNDPRRKPLDSRVLHAVKYYCQNFAPNFKESEMNAIAADMCTNARRVVRKSWMPKVKVLKAEDDAYTTFISETGKIEPDMMGVEHGFETASHEGEAGPSAEALQ

相同性解析による交差性が予測される生物種
Full Name nucleus accumbens associated 1, BEN and BTB (POZ) domain containing
Calculated molecular weight 57 kDa
Observed molecular weight60-65 kDa
GenBank accession numberBC055396
Gene Symbol NACC1
Gene ID (NCBI) 112939
RRIDAB_2918036
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96RE7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NACC1, also named as BTBD14B and NAC1, belongs to the BTB/POZ family. NACC1 is a transcriptional repressor, which is amplified and overexpressed in various human cancers and plays critical roles in tumor development, progression, and drug resistance (PMID: 31101655). NACC1 has 1 isoform with the molecular mass of 57 kDa. While NAC1 has been shown to repress the transcription of two tumor suppressors, Gadd45-g and Gadd45-g- interacting protein 1, scientists have found that NACC1 also plays an important role in nontranscriptional function, for example, it can act as a bridge for MAVS and TBK1 in RLR-mediated signaling (PMID: 31235549).

Protocols

Product Specific Protocols
IF protocol for NAC1 antibody 14551-1-APDownload protocol
IHC protocol for NAC1 antibody 14551-1-APDownload protocol
IP protocol for NAC1 antibody 14551-1-APDownload protocol
WB protocol for NAC1 antibody 14551-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Clin Transl Hepatol

Exosome-transported circHDAC1_004 Promotes Proliferation, Migration, and Angiogenesis of Hepatocellular Carcinoma by the miR-361-3p/NACC1 Axis

Authors - Bin Xu
View Article
humanWB

Oncol Rep

lncRNA FOXP4‑AS1 predicts poor prognosis and accelerates the progression of mantle cell lymphoma through the miR‑423‑5p/NACC1 pathway.

Authors - Hong-Fang Tao
View Article
humanWB

Genes Genomics

ST6GALNAC1 promotes the invasion and migration of breast cancer cells via the EMT pathway

Authors - Yunzhao Luo
View Article
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