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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using NACA Polyclonal antibody (32235-1-AP)

    WB analysis of HeLa using 32235-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 32235-1-AP (NACA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using NACA Polyclonal antibody (32235-1-AP)

    IHC staining of mouse kidney using 32235-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 32235-1-AP (NACA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using NACA Polyclonal antibody (32235-1-AP)

    IHC staining of mouse kidney using 32235-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 32235-1-AP (NACA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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NACA Polyclonal antibody

NACA Polyclonal Antibody for WB, IHC, ELISA
Cat No. 32235-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Alpha NAC, Hom s 2, HSD48, NAC alpha, NACA1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, U-87 MG cells, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32235-1-AP targets NACA in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NACA fusion protein Ag37704 相同性解析による交差性が予測される生物種
Full Name nascent polypeptide-associated complex alpha subunit
Calculated molecular weight 215 aa, 23 kDa
Observed molecular weight35 kDa
GenBank accession numberBC106041
Gene Symbol NACA
Gene ID (NCBI) 4666
RRIDAB_3670227
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ13765
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NACA (NAC-alpha), or Nascent polypeptide-associated complex subunit alpha, is a component of the nascent polypeptide-associated complex (NAC). NACA is a key subunit of the NAC complex that assists in the proper folding of newly synthesized proteins and prevents the formation of harmful protein aggregates, playing a vital role in cellular protein quality control and stress response mechanisms. The calculated MW of NACA is 23 kDa, and the observed MW is 35 kDa, which is due to phosphorylation modification (PMID: 30948508, 36107769).

Protocols

Product Specific Protocols
WB protocol for NACA antibody 32235-1-APDownload protocol
IHC protocol for NACA antibody 32235-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols