Validation Data Gallery

Filter: AllWB

WB analysis of human liver using 16954-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 16954-1-AP (N6AMT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 16954-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 16954-1-AP (N6AMT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 16954-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16954-1-AP (N6AMT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 16954-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 16954-1-AP (N6AMT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 16954-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 16954-1-AP (N6AMT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

N6AMT2 Polyclonal antibody

N6AMT2 Polyclonal Antibody for WB, ELISA

Cat No. 16954-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

EC:2.1.1.-, EEF1A lysine methyltransferase 1, eEF1A-KMT, EEF1AKMT1, ESP13

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in

human liver tissue, A431 cells, HEK-293 cells, mouse kidney tissue, mouse testis tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16954-1-AP targets N6AMT2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag10589 Product name: Recombinant human N6AMT2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-214 aa of BC018091 Sequence: MSDLEDDETPQLSAHALAALQEFYAEQKQQIEPGEDDKYNIGIIEENWQLSQFWYSQETALQLAQEAIAAVGEGGRIACVSAPSVYQKLRELCRENFSIYIFEYDKRFAMYGEEFIFYDYNNPLDLPERIAAHSFDIVIADPPYLSEECLRKTSETVKYLTRGKILLCTGAIMEEQAAELLGVKMCTFVPRHTRNLANEFRCYVNYDSGLDCGI 相同性解析による交差性が予測される生物種
Full Name	N-6 adenine-specific DNA methyltransferase 2 (putative)
Calculated molecular weight	214 aa, 25 kDa
Observed molecular weight	25 kDa
GenBank accession number	BC018091
Gene Symbol	N6AMT2
Gene ID (NCBI)	221143
RRID	AB_2282277
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8WVE0
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for N6AMT2 antibody 16954-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

製品

アプリケーション

cGMPグレード製品

サポート

カスタム対応

プロモーション

企業情報

抗体

免疫アッセイ

サイトカイン・増殖因子

ChromoTek VHH（Nanobody®）試薬

サポートツール・試薬

ゲノミクス試薬

研究分野

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of human liver using 16954-1-AP

WB analysis of A431 using 16954-1-AP

WB analysis of mouse kidney using 16954-1-AP

WB analysis of HEK-293 using 16954-1-AP

WB analysis of mouse testis using 16954-1-AP