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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using N-cadherin Monoclonal antibody (68532-2-Ig)

    WB analysis using 68532-2-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68532-2-Ig (N-cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using N-cadherin Monoclonal antibody (68532-2-Ig)

    IF Staining of HepG2 using 68532-2-Ig

    Immunofluorescent analysis of un-fixed HepG2 cells using N-cadherin antibody (68532-2-Ig, Clone: 2D9G1 ) at dilution of 1:500 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

  • Flow cytometry (FC) experiment of HepG2 cells using N-cadherin Monoclonal antibody (68532-2-Ig)

    FC experiment of HepG2 using 68532-2-Ig

    1x10^6 HepG2 cells were surface stained with 0.2 μg N-Cadherin Monoclonal Antibody (68532-2-Ig, Clone: 2D9G1, red) or 0.2 μg Mouse IgG1 isotype control (66360-1-Ig, Clone: 1F8D3, blue), and CoraLite488-conjugated Goat Anti-Mouse IgG(H+L) (Cat.NO. SA00013-1). Cells were not fixed.

  • Flow cytometry (FC) experiment of SH-SY5Y cells using N-cadherin Monoclonal antibody (68532-2-Ig)

    FC experiment of SH-SY5Y using 68532-2-Ig

    1x10^6 MCF-7 (red, dashed) and SH-SY5Y (red, filled) were surface stained with 0.2 μg N-cadherin Monoclonal antibody (68532-2-Ig, Clone: 2D9G1) and CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(Cat.NO.RGAM005). Mouse IgG1 isotype control (66360-1-Ig, Clone: 1F8D3, blue) was parallel stained as control. Cells were not fixed.

  • Affinity and Kinetic Characterization of 68532-2-Ig

    Affinity and Kinetic Characterization of 68532-2-Ig

    Biolayer interferometry (BLl) kinetic assays of 68532-2-PBS against Human N-cadherin were performed. The affinity constant is 2.69 nM.

N-cadherin Monoclonal antibody

N-cadherin Monoclonal Antibody for WB, IF/ICC, FC, ELISA
Cat No. 68532-2-Ig
Clone No.2D9G1

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IF/ICC, FC, ELISA

Synonyms

2D9G1, Cadherin 2, Cadherin-2, CD325, CDH2

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inpig heart tissue, rabbit heart tissue, rat heart tissue, mouse heart tissue
Positive IF/ICC detected inHepG2 cells
Positive FC detected inSH-SY5Y cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68532-2-Ig targets N-cadherin in WB, IF/ICC, FC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Eg0701

Product name: Recombinant Human N-Cadherin protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 160-724 aa of NM_001792.4

Sequence: DWVIPPINLPENSRGPFPQELVRIRSDRDKNLSLRYSVTGPGADQPPTGIFIINPISGQLSVTKPLDREQIARFHLRAHAVDINGNQVENPIDIVINVIDMNDNRPEFLHQVWNGTVPEGSKPGTYVMTVTAIDADDPNALNGMLRYRIVSQAPSTPSPNMFTINNETGDIITVAAGLDREKVQQYTLIIQATDMEGNPTYGLSNTATAVITVTDVNDNPPEFTAMTFYGEVPENRVDIIVANLTVTDKDQPHTPAWNAVYRISGGDPTGRFAIQTDPNSNDGLVTVVKPIDFETNRMFVLTVAAENQVPLAKGIQHPPQSTATVSVTVIDVNENPYFAPNPKIIRQEEGLHAGTMLTTFTAQDPDRYMQQNIRYTKLSDPANWLKIDPVNGQITTIAVLDRESPNVKNNIYNATFLASDNGIPPMSGTGTLQIYLLDINDNAPQVLPQEAETCETPDPNSINITALDYDIDPNAGPFAFDLPLSPVTIKRNWTITRLNGDFAQLNLKIKFLEAGIYEVPIIITDSGNPPKSNISILRVKVCQCDSNGDCTDVDRIVGAGLGTGA

相同性解析による交差性が予測される生物種
Full Name cadherin 2, type 1, N-cadherin (neuronal)
Calculated molecular weight100 kDa
Observed molecular weight130 kDa
GenBank accession numberNM_001792.4
Gene Symbol N-cadherin
Gene ID (NCBI) 1000
RRIDAB_3670382
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP19022-1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. N-cadherin (neural cadherin), also known as CDH2 (cadherin 2), is a 130-kDa transmembrane protein and a classical member of the cadherin superfamily which also include E-, P-, R-, and B-cadherins. Expression of N-cadherin has been reported on various cell types including neurons, endothelial cells and cardiac myocytes (PMID: 11282032; 9508779; 8125202). N-cadherin has functions in early brain morphogenesis, synaptogenesis and synaptic plasticity (PMID: 23321619). The N-cadherin ectodomain can be cleaved, leading to the generation of a 90 kD N-terminal fragment (PMID: 16998833; 17028923).

Protocols

Product Specific Protocols
IF protocol for N-cadherin antibody 68532-2-IgDownload protocol
WB protocol for N-cadherin antibody 68532-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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