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GLYR1 Polyclonal antibody

GLYR1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 14833-1-AP

Synonyms

NDF, hNDF, HIBDL, Glyoxylate reductase 1 homolog, Cytokine-like nuclear factor N-PAC


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Tested Applications

Positive WB detected inHL-60 cells, HeLa cells, Jurkat cells
Positive IP detected inK-562 cells
Positive IHC detected inmouse testis tissue, human liver cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14833-1-AP targets GLYR1 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GLYR1 fusion protein Ag6637 相同性解析による交差性が予測される生物種
Full Name cytokine-like nuclear factor n-pac
Calculated molecular weight 61 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC064940
Gene symbol N-PAC
Gene ID (NCBI) 84656
RRIDAB_10859775
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GLYR1 antibody 14833-1-APDownload protocol
IHC protocol for GLYR1 antibody 14833-1-APDownload protocol
IF protocol for GLYR1 antibody 14833-1-APDownload protocol
IP protocol for GLYR1 antibody 14833-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human

Cell

Transcription factor protein interactomes reveal genetic determinants in heart disease.

Authors - Barbara Gonzalez-Teran
mouseChIP, WB

Genomics Proteomics Bioinformatics

Npac Is a Co-factor of Histone H3K36me3 and Regulates Transcriptional Elongation in Mouse Embryonic Stem Cells.

Authors - Sue Yu
humanWB,IHC,CoIP,IF

J Exp Clin Cancer Res

Downregulation of GLYR1 contributes to microsatellite instability colorectal cancer by targeting p21 via the p38MAPK and PI3K/AKT pathways.

Authors - Zhiyan Hu
  • KD Validated