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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MultiProTM-5CFLX Anti-Human CD45 (HI30)

CD45 Monoclonal Antibody for

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

Conjugate

5CFLX Fluorescent Dye

CloneNo.

HI30

Cat no : G65109-1-5C

Synonyms

B220, CD45, CD45R, GP180, HI30, L CA, LCA, Leukocyte common antigen, LY5, PTPRC, RPTPC, T200

Validation Data Gallery

Applications:
  • Single Cell Sequencing experiment G65109-1-5C on Resting PBMC

    Single Cell Sequencing experiment G65109-1-5C on Resting PBMC

    G65109-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G65109-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G65109-1-5C on PBMC treated with 4hr LPS + TI

    G65109-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G65109-1-5C on Resting PBMC

    Single Cell Sequencing experiment G65109-1-5C on Resting PBMC

    G65109-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Stain-Fix protocol.

  • Single Cell Sequencing experiment G65109-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G65109-1-5C on PBMC treated with 4hr LPS + TI

    G65109-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Stain-Fix protocol.

Tested Applications

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product
Positive Single Cell detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product
Planning an IHC experiment? We recommend our IHCeasy CD45 Ready-To-Use IHC Kit. CD45 primary antibody included.

Recommended dilution

ApplicationDilution
SINGLE CELL (INTRA)SINGLE CELL (INTRA) : <0.5ug/test
SINGLE CELLSINGLE CELL : <0.5ug/test
Sample-dependent, check data in validation data gallery

Product Information

G65109-1-5C targets CD45 in applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen Human peripheral blood leucocytes 相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, receptor type, C
GenBank accession numberBC014239
Gene symbol PTPRC
Gene ID (NCBI) 5788
Conjugate 5CFLX Fluorescent Dye
Form Liquid
Purification Method
Storage Buffer PBS with 1mM EDTA and 0.09% sodium azide
Storage Conditions2-8°C

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).