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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MultiProTM-5CFLX Anti-Human CD43 (2A11D6)

CD43 Monoclonal Antibody for

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

Conjugate

5CFLX Fluorescent Dye

CloneNo.

2A11D6

Cat no : G66224-1-5C

Synonyms

CD43, Galactoglycoprotein, GALGP, GPL115, Leukocyte sialoglycoprotein, Leukosialin, LSN, sialophorin, SPN

Validation Data Gallery

Applications:
  • Single Cell Sequencing experiment G66224-1-5C on Resting PBMC

    Single Cell Sequencing experiment G66224-1-5C on Resting PBMC

    G66224-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G66224-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G66224-1-5C on PBMC treated with 4hr LPS + TI

    G66224-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

Tested Applications

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product
Planning an IHC experiment? We recommend our IHCeasy CD43 Ready-To-Use IHC Kit. CD43 primary antibody included.

Recommended dilution

ApplicationDilution
SINGLE CELL (INTRA)SINGLE CELL (INTRA) : <0.5ug/test
Sample-dependent, check data in validation data gallery

Product Information

G66224-1-5C targets CD43 in applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CD43 fusion protein Ag23595 相同性解析による交差性が予測される生物種
Full Name sialophorin
Calculated molecular weight 400 aa, 43 kDa
GenBank accession numberBC012350
Gene symbol SPN
Gene ID (NCBI) 6693
Conjugate 5CFLX Fluorescent Dye
Form Liquid
Purification Method
Storage Buffer PBS with 1mM EDTA and 0.09% sodium azide
Storage Conditions2-8°C

Background Information

CD43, also named leukosialin, sialophorin, galactoglycoprotein, leukocyte sialoglycoprotein, SPN, and is a mucin-like type I transmembrane protein. In humans, it is expressed in haematopoietic cells, including T lymphocytes, monocytes, granulocytes, natural killer cells, platelets, and haematopoietic stem cells, with the exception of mature erythrocytes and B cell subpopulations. The unglycosylated form of CD43 migrates on SDS-PAGE with a molecular weight of 54 kDa (PMID: 2943740), whereas CD43 from different haematopoietic cell lines displays increased molecular weights since it is glycosylated with O-linked chains that differ in core structure and sialylation.