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Validation Data Gallery

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  • Single Cell Sequencing experiment G65254-1-5C on Resting PBMC

    Single Cell Sequencing experiment G65254-1-5C on Resting PBMC

    G65254-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G65254-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G65254-1-5C on PBMC treated with 4hr LPS + TI

    G65254-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G65254-1-5C on Resting PBMC

    Single Cell Sequencing experiment G65254-1-5C on Resting PBMC

    G65254-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Stain-Fix protocol.

  • Single Cell Sequencing experiment G65254-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G65254-1-5C on PBMC treated with 4hr LPS + TI

    G65254-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Stain-Fix protocol.

MultiPro® 5CFLX Anti-Human CD35 (E11)

CD35 Monoclonal Antibody for Single Cell (Intra), Single Cell
Cat No. G65254-1-5C
Clone No.E11

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

Single Cell (Intra), Single Cell

Synonyms

C3b/C4b receptor, C3BR, CD35, Complement receptor type 1, CR1, KN

Formulation:  PBS, Azide
PBS, Azide
Conjugate:  5CFLX
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.
Positive Single Cell detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.

Recommended dilution

ApplicationDilution
SINGLE CELL (INTRA)<0.5ug/test
SINGLE CELL<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

G65254-1-5C targets CD35 in Single Cell (Intra), Single Cell applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1, kappa
Class Oligo Conjugate
Type Monoclonal
Immunogen

Human CD35 from Acute monocytic leukemia cells and normal blood monocytes

相同性解析による交差性が予測される生物種
Full Name MultiPro® 5CFLX Anti-Human CD35 (E11)
Calculated molecular weight 224 kDa
GenBank accession numberNM_000573
Gene Symbol CR1
Gene ID (NCBI) 1378
ENSEMBL Gene IDENSG00000203710
RRIDAB_3673940
Conjugate 5CFLX
Full Oligo SequenceCGGAGATGTGTATAAGAGACAGAATGAATTGGATTCGCCCATATAAGAAA
Barcode SequenceAATGAATTGGATTCG
Form
FormLiquid
UNIPROT IDP17927
Storage Buffer PBS with 1mM EDTA and 0.09% sodium azide {{ptg:BufferTemp}}7.3
Storage Conditions2-8°C Stable for one year after shipment.

Background Information

CD35, also known as complement receptor type 1 (CR1) or C3b/C4b receptor, is a single-chain glycoprotein consisting of 30 repeating homologous protein domains known as short consensus repeats (~60 amino acids each) followed by transmembrane and cytoplasmic domains (PMID: 8765013). CD35 is variably expressed by granulocytes, monocytes, B cells, some T cells, erythrocytes, follicular dendritic cells, Langerhans cells, and glomerular podocytes (PMID: 19004497). CD35 acts as a receptor for C3b and C4b and plays important roles in the regulation of the complement cascade and clearance of immune complexes.

Protocols

MultiPro™ Cell Surface and Intracellular Staining ProtocolDownload protocol
10x Genomics Cell Surface Protein Only Staining ProtocolDownload protocol
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