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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MultiProTM-5CFLX Anti-Human CD3 (OKT3)

CD3 Monoclonal Antibody for

Host / Isotype

Mouse / IgG2a, kappa

Reactivity

Human

Applications

Conjugate

5CFLX Fluorescent Dye

CloneNo.

OKT3

Cat no : G65133-1-5C

Synonyms

CD3, CD3E, T3E, TCRE

Validation Data Gallery

Applications:
  • Single Cell Sequencing experiment G65133-1-5C on Resting PBMC

    Single Cell Sequencing experiment G65133-1-5C on Resting PBMC

    G65133-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G65133-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G65133-1-5C on PBMC treated with 4hr LPS + TI

    G65133-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

Tested Applications

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product
Planning an IHC experiment? We recommend our IHCeasy CD3 Ready-To-Use IHC Kit. CD3 primary antibody included.

Recommended dilution

ApplicationDilution
SINGLE CELL (INTRA)SINGLE CELL (INTRA) : <0.5ug/test
Sample-dependent, check data in validation data gallery

Product Information

G65133-1-5C targets CD3 in applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a, kappa
Class Monoclonal
Type Antibody
Immunogen Human T cells 相同性解析による交差性が予測される生物種
Full Name CD3e molecule, epsilon (CD3-TCR complex)
Calculated molecular weight 207 aa, 23 kDa
GenBank accession numberBC049847
Gene symbol CD3E
Gene ID (NCBI) 916
Conjugate 5CFLX Fluorescent Dye
Form Liquid
Purification Method
Storage Buffer PBS with 1mM EDTA and 0.09% sodium azide
Storage Conditions2-8°C

Background Information

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).