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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MultiProTM-5CFLX Anti-Human CD27 (1C1G3)

CD27 Monoclonal Antibody for

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

Conjugate

5CFLX Fluorescent Dye

CloneNo.

1C1G3

Cat no : G66308-1-5C

Synonyms

CD27, CD27 antigen, CD27 molecule, CD27L receptor, S152, T cell activation antigen CD27, T14, TNFRSF7, Tp55

Validation Data Gallery

Applications:
  • Single Cell Sequencing experiment G66308-1-5C on Resting PBMC

    Single Cell Sequencing experiment G66308-1-5C on Resting PBMC

    G66308-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G66308-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G66308-1-5C on PBMC treated with 4hr LPS + TI

    G66308-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G66308-1-5C on Resting PBMC

    Single Cell Sequencing experiment G66308-1-5C on Resting PBMC

    G66308-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Stain-Fix protocol.

  • Single Cell Sequencing experiment G66308-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G66308-1-5C on PBMC treated with 4hr LPS + TI

    G66308-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Stain-Fix protocol.

Tested Applications

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product
Positive Single Cell detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product

Recommended dilution

ApplicationDilution
SINGLE CELL (INTRA)SINGLE CELL (INTRA) : <0.5ug/test
SINGLE CELLSINGLE CELL : <0.5ug/test
Sample-dependent, check data in validation data gallery

Product Information

G66308-1-5C targets CD27 in applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD27 fusion protein Ag24537 相同性解析による交差性が予測される生物種
Full Name CD27 molecule
Calculated molecular weight 29 kDa
GenBank accession numberBC012160
Gene symbol CD27
Gene ID (NCBI) 939
Conjugate 5CFLX Fluorescent Dye
Form Liquid
Purification Method
Storage Buffer PBS with 1mM EDTA and 0.09% sodium azide
Storage Conditions2-8°C

Background Information

CD27 (also known as TNFRSF7) is a type I glycoprotein expressed on some B cells and the majority of T cells, and is a member of the tumor necrosis factor (TNF) receptor family. CD27 is required for generation and long-term maintenance of T cell immunity (PMID: 11062504). It is a receptor for CD70 (CD27L). Ligation of CD27 by CD70 induces strong ubiquitination of TRAF and the activation of both canonical and non-canonical NF-kappaB pathways, as well as the JNK pathway (PMID: 19426224). CD27 may also play a role in apoptosis through association with SIVA1.