Validation Data Gallery
Tested Applications
| Positive Single Cell (Intra) detected in | 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product. |
| Positive Single Cell detected in | 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product. |
Recommended dilution
| Application | Dilution |
|---|---|
| SINGLE CELL (INTRA) | <0.5ug/test |
| SINGLE CELL | <0.5ug/test |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
G65110-1-5C targets CD19 in Single Cell (Intra), Single Cell applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Oligo Conjugate |
| Type | Monoclonal |
| Immunogen |
N/A 相同性解析による交差性が予測される生物種 |
| Full Name | MultiPro® 5CFLX Anti-Human CD19 (HIB19) |
| Calculated molecular weight | 556 aa, 61 kDa |
| GenBank accession number | BC006338 |
| Gene Symbol | CD19 |
| Gene ID (NCBI) | 930 |
| ENSEMBL Gene ID | ENSG00000177455 |
| RRID | AB_3673915 |
| Conjugate | 5CFLX |
| Full Oligo Sequence | CGGAGATGTGTATAAGAGACAGCGGCTGGTCCTTCATCCCATATAAGAAA |
| Barcode Sequence | CGGCTGGTCCTTCAT |
| Form | |
| Form | Liquid |
| Storage Buffer | PBS with 1mM EDTA and 0.09% sodium azide {{ptg:BufferTemp}}7.3 |
| Storage Conditions | 2-8°C Stable for one year after shipment. |
Background Information
CD19 is a 95 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily (PMID: 2472450). It is expressed by B cells and follicular dendritic cells. CD19 is up-regulated at the step of B-lineage commitment during the differentiation of the hematopoietic stem cell, it remains on during subsequent stages of differentiation until finally down-regulated during terminal differentiation into plasma cells (PMID: 8528044). CD19 is involved in B cell development, activation and differentiation. It is the dominant component for the signaling complex on B cells that includes CD21 (CR2), CD81 (TAPA-1) and CD225 and acts as a critical co-receptor for BCR signal transduction (PMID: 23210908).
Protocols
| MultiPro™ Cell Surface and Intracellular Staining Protocol | Download protocol |
| 10x Genomics Cell Surface Protein Only Staining Protocol | Download protocol |
