Recombinant Mouse VISTA protein (mFc Tag)(HPLC verified)
Species
Mouse
Purity
>90 %, SDS-PAGE
>90%, SEC-HPLC
Tag
mFc Tag
Activity
not tested
Cat no : Eg4197
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE >90%, SEC-HPLC |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Human VISTA protein Phe33-Ala191 (Accession# Q9D659) with a mouse IgG Fc tag at the C-terminus. |
| GeneID | 74048 |
| Accession | Q9D659 |
| PredictedSize | 44.4 kDa |
| SDS-PAGE | 50-65 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
VISTA, also known as PD-1H, Dies1, and Gi24, is encoded by the Vsir gene in mice. VISTA is a type I transmembrane protein that acts as an immune-regulatory checkpoint on hematopoietic cells and shares homology with both the B7 family ligand PD-L1 and the CD28 family receptor PD-1. In mice, VISTA is expressed predominantly in hematopoietic tissues, such as the spleen, thymus, lymph node, and bone marrow (BM). VISTA plays a key role in regulating immune system responses and maintaining homeostasis.
References:
1.Yuan L, et al. (2021) Trends Immunol. 42(3):209-227. 2.Tagliamento M, et al. (2021) Immunotargets Ther. 10:185-200. 3.Martin AS, et al. (2023) Front Immunol. 14:1086102. 4.Shekari N, et al. (2023) Cancer Cell Int. 23(1):265. 5.Zhang RJ, et al. (2024) Exp Mol Med. 56(11):2348-2356.