Speedy™ Mouse VEGF One-Step ELISA Kit
Sensitivity
0.6 pg/mL
Range
15.6-1000 pg/mL
Reactivity
Mouse
Cat no : SE60009
Synonyms
Vegfa, L-VEGF , N-VEGF, Vascular Endothelial Growth Factor, vascular endothelial growth factor A
Validation Data Gallery
Product Information
| Product name | Speedy™ Mouse VEGF One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 0.6 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 22339 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 86% | 83%-91% |
| Cell culture supernatant | 103% | 94%-111% |
| Tissue lysate | 84% | 72%-107% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 565.6 | 36.2 | 6.4 |
| 2 | 8 | 124.5 | 5.2 | 4.2 |
| 3 | 8 | 42.6 | 2.1 | 4.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 573.7 | 49.3 | 8.6 |
| 2 | 16 | 124.0 | 5.7 | 4.6 |
| 3 | 16 | 42.7 | 1.9 | 4.4 |
Background Information
Vascular endothelial growth factor (VEGF), is a signal protein produced by cells that stimulates vasculogenesis and angiogenesis. It is part of the system that restores the oxygen supply to tissues when blood circulation is inadequate such as in hypoxic conditions. Serum concentration of VEGF is high in bronchial asthma and diabetes mellitus. The activities of VEGF are not limited to the vascular system; VEGF plays a role in normal physiological functions such as bone formation, hematopoiesis, wound healing, and development. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. VEGF is upregulated in many known tumors and its expression is correlated with tumor stage and progression.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Vegfa, L-VEGF , N-VEGF, Vascular Endothelial Growth Factor, vascular endothelial growth factor A |