Speedy™ Mouse VCAM-1/CD106 One-Step ELISA Kit
Sensitivity
5.6 pg/mL
Range
23.4-1500 pg/mL
Reactivity
Mouse
Cat no : SE60020
Synonyms
CD106, Vcam1, VCAM-1, Vcam 1
Validation Data Gallery
Product Information
| Product name | Speedy™ Mouse VCAM-1/CD106 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Urine |
| Assay type | Sandwich |
| Sensitivity | 5.6 pg/mL |
| Range | 23.4-1500 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 22329 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 105% | 101%-115% |
| Urine | 121% | 113%-129% |
| Cell culture supernatant | 110% | 96%-115% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 919.9 | 37.7 | 4.1 |
| 2 | 8 | 225.6 | 4.6 | 2.0 |
| 3 | 8 | 116.5 | 8.8 | 7.6 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 911.9 | 30.6 | 3.4 |
| 2 | 16 | 225.7 | 7.4 | 3.3 |
| 3 | 16 | 119.4 | 9.5 | 8.0 |
Background Information
Vascular cell adhesion molecule 1 (VCAM-1), also known as CD106, is a transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. VCAM-1 is expressed by cytokine-activated endothelium, interacts with integrin VLA4 (α4β1) present on the surface of leukocytes, and mediates both adhesion and signal transduction. It is also expressed either constitutively or inducibly in a variety of other cell types, including vascular smooth muscle cells, differentiating skeletal muscle cells, renal and neural epithelial cells, macrophages (Kupffer cells), dendritic cells, and bone marrow stromal cells. Soluble VCAM-1 (sVCAM-1) has been found in addition to membrane-bound VCAM-1. Increased sVCAM-1 has been shown to correlate with disease activity in various entities including rheumatoid arthritis, systemic lupus erythematosus, and lupus nephritis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD106, Vcam1, VCAM-1, Vcam 1 |